Label free and amplified detection of cancer marker EBNA-1 by DNA probe based biosensors

Gaizka Garai-Ibabe, Ruta Grinyte, Efim I. Golub, Allon Canaan, Marc Lamy de la Chapelle, Robert S. Marks, Valeri Pavlov

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Epstein-Barr virus (EBV) is a human herpes virus that has been associated with several malignancies as Burkitt's lymphoma, nasopharyngeal carcinoma and Hodgkin's disease. All EBV associated malignancies showed a distinct viral gene expression pattern, while Epstein-Barr nuclear antigen 1 (EBNA-1) is constitutively expressed in all such disorders. Here, the development of a biosensor to detect EBNA-1 protein is reported, which was based on a nucleic acid bioreceptor and a quartz crystal microbalance with a dissipation monitoring (QCM-D) transducer. The DNA probe for EBNA-1 detection was designed and synthesized to mimic its palindromic target sites in the EBV genome. This DNA probe was immobilized on the Au-surface of a QCM-D electrode, followed by the blocking of the accessible Au-surface with 6-mercapto-1-hexanol (6-MHO). The system showed a limit of detection of 50. ng/mL in direct detection of EBNA-1, however, the sensitivity was improved by 2 orders of magnitude (0.5. ng/mL) when an amplification cascade, employing antibodies labeled with alkaline phosphatase (AP), was applied to the system.

Original languageEnglish
Pages (from-to)272-275
Number of pages4
JournalBiosensors and Bioelectronics
Volume30
Issue number1
DOIs
StatePublished - 15 Dec 2011

Keywords

  • Biosensor
  • DNA
  • EBNA-1
  • Epstein-Barr virus
  • Quartz crystal microbalance (QCM-D)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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