Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Stefan Jaric, Aabha Bajaj, Vladimir Vukic, Ivana Gadjanski, Ibrahim Abdulhalim, Ivan Bobrinetskiy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer’s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.

Original languageEnglish
Article number326
JournalToxins
Volume15
Issue number5
DOIs
StatePublished - 1 May 2023

Keywords

  • CVD graphene
  • aptamer
  • cyanotoxins
  • label-free biosensing
  • surface plasmon resonance

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

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