Abstract
A sensitive; simple and label-free method for DNA detection is presented. Irradiated polyethylene terephthalate foils were etched to present conical tracks at a density of approximately 5×107 tracks per cm2. Probe DNA was immobilized at the narrow opening of the tracks (2-4nm diameter). The presence of target DNA resulted in changes in both conductance and rectification ratios in a concentration-dependent manner. DNA concentrations as low as 2nM could be detected using this simple two-step detection scheme.
Original language | English |
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Pages (from-to) | 362-366 |
Number of pages | 5 |
Journal | Biosensors and Bioelectronics |
Volume | 42 |
Issue number | 1 |
DOIs | |
State | Published - 5 Apr 2013 |
Keywords
- Conical nanopores
- DNA bio-sensing
- H1N1 detection
- Ion tracks
- Label-free detection
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry