Label-free DNA detection using the narrow side of funnel-type etched nanopores

Yohai Mandabi; Dietmar Fink; Lital Alfonta

doi:10.1016/j.bios.2012.10.036

Label-free DNA detection using the narrow side of funnel-type etched nanopores

Yohai Mandabi, Dietmar Fink, Lital Alfonta

Department of Biotechnology Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A sensitive; simple and label-free method for DNA detection is presented. Irradiated polyethylene terephthalate foils were etched to present conical tracks at a density of approximately 5×10⁷ tracks per cm². Probe DNA was immobilized at the narrow opening of the tracks (2-4nm diameter). The presence of target DNA resulted in changes in both conductance and rectification ratios in a concentration-dependent manner. DNA concentrations as low as 2nM could be detected using this simple two-step detection scheme.

Original language	English
Pages (from-to)	362-366
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	42
Issue number	1
DOIs	https://doi.org/10.1016/j.bios.2012.10.036
State	Published - 5 Apr 2013

Keywords

Conical nanopores
DNA bio-sensing
H1N1 detection
Ion tracks
Label-free detection

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2012.10.036

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title = "Label-free DNA detection using the narrow side of funnel-type etched nanopores",

abstract = "A sensitive; simple and label-free method for DNA detection is presented. Irradiated polyethylene terephthalate foils were etched to present conical tracks at a density of approximately 5×107 tracks per cm2. Probe DNA was immobilized at the narrow opening of the tracks (2-4nm diameter). The presence of target DNA resulted in changes in both conductance and rectification ratios in a concentration-dependent manner. DNA concentrations as low as 2nM could be detected using this simple two-step detection scheme.",

keywords = "Conical nanopores, DNA bio-sensing, H1N1 detection, Ion tracks, Label-free detection",

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AU - Fink, Dietmar

AU - Alfonta, Lital

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Y1 - 2013/4/5

N2 - A sensitive; simple and label-free method for DNA detection is presented. Irradiated polyethylene terephthalate foils were etched to present conical tracks at a density of approximately 5×107 tracks per cm2. Probe DNA was immobilized at the narrow opening of the tracks (2-4nm diameter). The presence of target DNA resulted in changes in both conductance and rectification ratios in a concentration-dependent manner. DNA concentrations as low as 2nM could be detected using this simple two-step detection scheme.

AB - A sensitive; simple and label-free method for DNA detection is presented. Irradiated polyethylene terephthalate foils were etched to present conical tracks at a density of approximately 5×107 tracks per cm2. Probe DNA was immobilized at the narrow opening of the tracks (2-4nm diameter). The presence of target DNA resulted in changes in both conductance and rectification ratios in a concentration-dependent manner. DNA concentrations as low as 2nM could be detected using this simple two-step detection scheme.

KW - Conical nanopores

KW - DNA bio-sensing

KW - H1N1 detection

KW - Ion tracks

KW - Label-free detection

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JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

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ER -

Label-free DNA detection using the narrow side of funnel-type etched nanopores

Abstract

Keywords

ASJC Scopus subject areas

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