Label-free DNA detection using the narrow side of funnel-type etched nanopores

Yohai Mandabi, Dietmar Fink, Lital Alfonta

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A sensitive; simple and label-free method for DNA detection is presented. Irradiated polyethylene terephthalate foils were etched to present conical tracks at a density of approximately 5×107 tracks per cm2. Probe DNA was immobilized at the narrow opening of the tracks (2-4nm diameter). The presence of target DNA resulted in changes in both conductance and rectification ratios in a concentration-dependent manner. DNA concentrations as low as 2nM could be detected using this simple two-step detection scheme.

Original languageEnglish
Pages (from-to)362-366
Number of pages5
JournalBiosensors and Bioelectronics
Volume42
Issue number1
DOIs
StatePublished - 5 Apr 2013

Keywords

  • Conical nanopores
  • DNA bio-sensing
  • H1N1 detection
  • Ion tracks
  • Label-free detection

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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