Larvicidal activity of silver nanoparticles synthesized using Plumeria rubra plant latex against Aedes aegypti and Anopheles stephensi

Chandrashekhar D. Patil, Satish V. Patil, Hemant P. Borase, Bipinchandra K. Salunke, Rahul B. Salunkhe

Research output: Contribution to journalArticlepeer-review

173 Scopus citations

Abstract

In the present study activity of silver nano-particles (AgNPs) synthesized using Plumeria rubra plant latex against second and fourth larval instar of Aedes aegypti and Anopheles stephensi was determined. Range of concentrations of synthesized AgNps (10, 5, 2.5, 1.25, 0.625, 0.3125 ppm) and aqueous crude latex (1,000, 500, 250, 125, 62.50, 31.25 ppm) were tested against larvae of A. aegypti and A. Stephensi. The synthesized AgNps from P. rubra latex were highly toxic than crude latex extract in both mosquito species. The LC 50 values for second and fourth larval instars after 24 h of crude latex exposure were 1.49, 1.82 ppm against A. aegypti and 1.10, 1.74 ppm against A. stephensi respectively. These figures were 181.67, 287.49 ppm against A. aegypti and 143.69, 170.58 ppm against A. stephensi respectively for crude latex extract. The mortality rates were positively correlated with the concentration of AgNPs. The characterization studies of synthesized AgNPs by UV-Vis spectrophotometry, transmission electron microscopy (TEM), Particle size analysis (PSA) and zeta potential confirmed the spherical shape and size (32-200 nm) of silver nanoparticles alongwith stability. Toxicity studies carried out against non-target fish species Poecilia reticulata, the most common organism in the habitats of A. aegypti and A. stephensi showed no toxicity at LC 50 and LC 90 doses of the AgNPs. This is the first report on mosquito larvicidal activity of latex synthesized nanoparticles.

Original languageEnglish
Pages (from-to)1815-1822
Number of pages8
JournalParasitology Research
Volume110
Issue number5
DOIs
StatePublished - 1 May 2012
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • General Veterinary
  • Insect Science
  • Infectious Diseases

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