Abstract
Background fluorescence from serum chromophores is substantially reduced by a laserphotobleaching method. Human and bovine serum samples were illuminated with 337-nm light from a pulsed N2 laser for a short period of time. The serum emission in the region of 440 to 550 nm was reduced by an order of magnitude with no evident damage to serum proteins as judged by the unchanged activity of alkaline phosphotase and aspartate aminotransferase.
Original language | English |
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Pages (from-to) | 386-389 |
Number of pages | 4 |
Journal | Analytical Biochemistry |
Volume | 138 |
Issue number | 2 |
DOIs | |
State | Published - 1 May 1984 |
Keywords
- blood serum
- fluorescence immunoassays
- laser photobleaching
- photolysis
- serum fluorescence
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology