Laser photobleaching of human serum: Application to fluorescence immunoassays

Abraham H. Parola, Egidijus E. Uzgiris

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background fluorescence from serum chromophores is substantially reduced by a laserphotobleaching method. Human and bovine serum samples were illuminated with 337-nm light from a pulsed N2 laser for a short period of time. The serum emission in the region of 440 to 550 nm was reduced by an order of magnitude with no evident damage to serum proteins as judged by the unchanged activity of alkaline phosphotase and aspartate aminotransferase.

Original languageEnglish
Pages (from-to)386-389
Number of pages4
JournalAnalytical Biochemistry
Volume138
Issue number2
DOIs
StatePublished - 1 May 1984

Keywords

  • blood serum
  • fluorescence immunoassays
  • laser photobleaching
  • photolysis
  • serum fluorescence

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