@inproceedings{3bd727d0e7374488b53d06da37413eb6,
title = "Laser speckle time-series correlation analysis for bacteria activity detection",
abstract = "The study aims at development and laboratory approbation of non-contact optical technique for early evaluation of microbial activity. Microorganisms' activity is estimated by laser speckle contrast imaging technique in combination with image processing of obtained time varying speckle patterns. Laser speckle patterns were captured by CMOS sensor during illumination of growing bacteria colonies by low power (<30 mW, 635 nm) stabilized coherent light source. To validate proposed technique and image processing algorithm the vibrio natriegens bacteria are used. After analysis of several different experiments the following results were obtained: In the central part of the colony activity can be seen in 2.5-3 hours. Thus, earlier detection of bacterial activity is expected, i.e., earlier than 2-3 hours, which is much earlier than the standard counting methods used to count colony forming units (CFU).",
keywords = "Image processing, Laser speckle imaging, Microorganism activity estimation",
author = "Ilya Balmages and Dmitrijs Bliznuks and Janis Liepins and Stivens Zolins and Alexey Lihachev",
note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Biomedical Spectroscopy, Microscopy, and Imaging 2020 ; Conference date: 06-04-2020 Through 10-04-2020",
year = "2020",
month = jan,
day = "1",
doi = "10.1117/12.2541663",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Jurgen Popp and Csilla Gergely",
booktitle = "Biomedical Spectroscopy, Microscopy, and Imaging",
address = "United States",
}