Leishmania major: Excreted factor, calcium ions, and the survival of amastigotes

Yael Eilam; Joseph El-On; Dan T. Spira

doi:10.1016/0014-4894(85)90068-2

Leishmania major: Excreted factor, calcium ions, and the survival of amastigotes

Yael Eilam, Joseph El-On, Dan T. Spira

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Mouse macrophages infected with amastigotes of Leishmania major contain about 40% more intracellular exchangeable calcium than control macrophages. Similar elevation of intracellular exchangeable calcium was observed in macrophages engulfing red blood cells coated with purified excreted factor from L. major. The rate of cytolysis of red blood cells coated with excreted factor was significantly lower than that of uncoated controls. Excreted factor strongly binds calcium; thus, the possible role of a microenvironment rich in calcium bound to excreted factor within the phagolysosome in protecting the amastigotes may be considered.

Original language	English
Pages (from-to)	161-168
Number of pages	8
Journal	Experimental Parasitology
Volume	59
Issue number	2
DOIs	https://doi.org/10.1016/0014-4894(85)90068-2
State	Published - 1 Jan 1985
Externally published	Yes

Keywords

Amastigote
Calcium ions
Cytolysis
Hemoflagellate
Leishmania major
Macrophage
Mouse
Phagolysosomes
Protozoa, parasitic

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

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10.1016/0014-4894(85)90068-2

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title = "Leishmania major: Excreted factor, calcium ions, and the survival of amastigotes",

abstract = "Mouse macrophages infected with amastigotes of Leishmania major contain about 40% more intracellular exchangeable calcium than control macrophages. Similar elevation of intracellular exchangeable calcium was observed in macrophages engulfing red blood cells coated with purified excreted factor from L. major. The rate of cytolysis of red blood cells coated with excreted factor was significantly lower than that of uncoated controls. Excreted factor strongly binds calcium; thus, the possible role of a microenvironment rich in calcium bound to excreted factor within the phagolysosome in protecting the amastigotes may be considered.",

keywords = "Amastigote, Calcium ions, Cytolysis, Hemoflagellate, Leishmania major, Macrophage, Mouse, Phagolysosomes, Protozoa, parasitic",

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T2 - Excreted factor, calcium ions, and the survival of amastigotes

AU - Eilam, Yael

AU - El-On, Joseph

AU - Spira, Dan T.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Mouse macrophages infected with amastigotes of Leishmania major contain about 40% more intracellular exchangeable calcium than control macrophages. Similar elevation of intracellular exchangeable calcium was observed in macrophages engulfing red blood cells coated with purified excreted factor from L. major. The rate of cytolysis of red blood cells coated with excreted factor was significantly lower than that of uncoated controls. Excreted factor strongly binds calcium; thus, the possible role of a microenvironment rich in calcium bound to excreted factor within the phagolysosome in protecting the amastigotes may be considered.

AB - Mouse macrophages infected with amastigotes of Leishmania major contain about 40% more intracellular exchangeable calcium than control macrophages. Similar elevation of intracellular exchangeable calcium was observed in macrophages engulfing red blood cells coated with purified excreted factor from L. major. The rate of cytolysis of red blood cells coated with excreted factor was significantly lower than that of uncoated controls. Excreted factor strongly binds calcium; thus, the possible role of a microenvironment rich in calcium bound to excreted factor within the phagolysosome in protecting the amastigotes may be considered.

KW - Amastigote

KW - Calcium ions

KW - Cytolysis

KW - Hemoflagellate

KW - Leishmania major

KW - Macrophage

KW - Mouse

KW - Phagolysosomes

KW - Protozoa, parasitic

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ER -

Leishmania major: Excreted factor, calcium ions, and the survival of amastigotes

Abstract

Keywords

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