Leishmanial excreted factors (EFs): Purification by affinity chromatography

Uri Zehavi; Judith C. Abrahams; Ruth Granoth; Charles L. Greenblatt; Gerald M. Slutzky; Joseph El-On

doi:10.1007/BF00927419

Leishmanial excreted factors (EFs): Purification by affinity chromatography

Uri Zehavi, Judith C. Abrahams, Ruth Granoth, Charles L. Greenblatt, Gerald M. Slutzky, Joseph El-On

Research output: Contribution to journal › Article › peer-review

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Abstract

Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.

Original language	English
Pages (from-to)	695-701
Number of pages	7
Journal	Parasitology Research
Volume	69
Issue number	6
DOIs	https://doi.org/10.1007/BF00927419
State	Published - 1 Nov 1983
Externally published	Yes

ASJC Scopus subject areas

Parasitology

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10.1007/BF00927419

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title = "Leishmanial excreted factors (EFs): Purification by affinity chromatography",

abstract = "Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.",

author = "Uri Zehavi and Abrahams, {Judith C.} and Ruth Granoth and Greenblatt, {Charles L.} and Slutzky, {Gerald M.} and Joseph El-On",

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T2 - Purification by affinity chromatography

AU - Zehavi, Uri

AU - Abrahams, Judith C.

AU - Granoth, Ruth

AU - Greenblatt, Charles L.

AU - Slutzky, Gerald M.

AU - El-On, Joseph

PY - 1983/11/1

Y1 - 1983/11/1

N2 - Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.

AB - Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.

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DO - 10.1007/BF00927419

M3 - Article

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JO - Parasitology Research

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ER -

Leishmanial excreted factors (EFs): Purification by affinity chromatography

Abstract

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