Leishmanial excreted factors (EFs): Purification by affinity chromatography

Uri Zehavi, Judith C. Abrahams, Ruth Granoth, Charles L. Greenblatt, Gerald M. Slutzky, Joseph El-On

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.

Original languageEnglish
Pages (from-to)695-701
Number of pages7
JournalParasitology Research
Issue number6
StatePublished - 1 Nov 1983
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology


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