Lensless digital holographic microscope for label-free imaging

Manoj Kumar, Osamu Matoba, Mitsuhiro Morita, Yasuhiro Awatsuji

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


A new configuration of lensless digital holographic microscope based on a wedge plate is demonstrated. The system is simple in geometry, compact in size and highly stable in temporal phase measurements leading to the accurate investigations of minute membrane fluctuations of the biological cells. The proposed system is used for label-free quantitative phase imaging of the human red blood cells (RBCs) and the investigations of morphology and physiological parameters related to the phase information. The obtained results confirm the feasibility and effectiveness of the proposed system.

Original languageEnglish
Title of host publicationBiomedical Imaging and Sensing Conference 2021
EditorsToyohiko Yatagai, Yoshihisa Aizu, Osamu Matoba, Yasuhiro Awatsuji, Yuan Luo
ISBN (Electronic)9781510647190
StatePublished - 1 Jan 2021
Externally publishedYes
EventBiomedical Imaging and Sensing Conference 2021 - Virtual, Online, Japan
Duration: 20 Apr 202122 Apr 2021

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceBiomedical Imaging and Sensing Conference 2021
CityVirtual, Online


  • Digital holographic microscopy
  • Label-free phase imaging
  • Lensless imaging
  • Red blood cells

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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