TY - JOUR
T1 - Lentiviral-Transduced Ectopic Expression of Androgenic Hormone in a Crustacean Hematopoietic Primary Cell Culture
AU - Rotem-Dai, Noa
AU - Weil, Simy
AU - Greenshpan, Yariv
AU - Abehsera, Shai
AU - Manor, Rivka
AU - Ofir, Rivka
AU - Gazit, Roi
AU - Rosental, Benyamin
AU - Aflalo, Eliahu D.
AU - Sagi, Amir
N1 - Funding Information:
This work was supported by the Israel Ministry of Science, Technology and Space (Grant No. 13610-3 to NRD). The work of BR was supported by the Israel Science Foundation (ISF, Grants Nos. 1416/19 and 2841/19). Further partial support was provided by Enzootic Genetics and Innovation, the Israel Science Foundation (ISF) within the ISF-UGC joint research program framework (Grant No. 2728/2016), and the ISF-NSFC joint research program (Grant No. 2368/2018).
Publisher Copyright:
© Copyright © 2021 Rotem-Dai, Weil, Greenshpan, Abehsera, Manor, Ofir, Gazit, Rosental, Aflalo and Sagi.
PY - 2021/10/14
Y1 - 2021/10/14
N2 - Novel monosex biotechnologies in crustacean aquaculture involve the induction of sex reversal through manipulations of the androgenic gland (AG), and its most prominent factor, the insulin-like androgenic gland (IAG) hormone, during early developmental stages. In the giant freshwater prawn Macrobrachium rosenbergii, all-female populations are desirable for aquaculture, since the females can be stocked at higher densities and exhibit more uniform growth than males. Recently, a novel biotechnology for all-female aquaculture was developed based on injection into post-larvae of suspended primary AG cells from mature M. rosenbergii males. However, this biotechnology depends on the availability of appropriate male donors and it requires delicate surgical procedures on the small endocrine AG to produce appropriate quantities of cells for the sex manipulation. We therefore established a new platform for the production of M. rosenbergii IAG (Mr-IAG) in hematopoietic cells. The method rests on the induction of ectopic Mr-IAG expression under the white spot syndrome virus (WSSV) immediate-early 1 (IE1) promoter via a novel lentiviral vector. Our results demonstrate that M. rosenbergii primary cells infected with the Mr-IAG lentiviruses are capable of transcription, translation, and secretion of Mr-IAG in culture. Our new platform, which produces easy-to-harvest cells in abundance, could replace the AG cells used in the first step of the above-mentioned biotechnology for all-female aquaculture and, importantly, pave the way for producing monosex populations in other edible crustacean aquaculture species. In addition, a lentiviral system for crustacean cells provides a useful tool for basic and applied research in crustacean species.
AB - Novel monosex biotechnologies in crustacean aquaculture involve the induction of sex reversal through manipulations of the androgenic gland (AG), and its most prominent factor, the insulin-like androgenic gland (IAG) hormone, during early developmental stages. In the giant freshwater prawn Macrobrachium rosenbergii, all-female populations are desirable for aquaculture, since the females can be stocked at higher densities and exhibit more uniform growth than males. Recently, a novel biotechnology for all-female aquaculture was developed based on injection into post-larvae of suspended primary AG cells from mature M. rosenbergii males. However, this biotechnology depends on the availability of appropriate male donors and it requires delicate surgical procedures on the small endocrine AG to produce appropriate quantities of cells for the sex manipulation. We therefore established a new platform for the production of M. rosenbergii IAG (Mr-IAG) in hematopoietic cells. The method rests on the induction of ectopic Mr-IAG expression under the white spot syndrome virus (WSSV) immediate-early 1 (IE1) promoter via a novel lentiviral vector. Our results demonstrate that M. rosenbergii primary cells infected with the Mr-IAG lentiviruses are capable of transcription, translation, and secretion of Mr-IAG in culture. Our new platform, which produces easy-to-harvest cells in abundance, could replace the AG cells used in the first step of the above-mentioned biotechnology for all-female aquaculture and, importantly, pave the way for producing monosex populations in other edible crustacean aquaculture species. In addition, a lentiviral system for crustacean cells provides a useful tool for basic and applied research in crustacean species.
KW - androgenic gland (AG)
KW - hematopoietic cells
KW - insulin-like androgenic gland switch (IAG-switch)
KW - lentivirus
KW - Macrobrachium rosenbergii
KW - monosex aquaculture
KW - white spot syndrome virus
UR - http://www.scopus.com/inward/record.url?scp=85118234624&partnerID=8YFLogxK
U2 - 10.3389/fmars.2021.677679
DO - 10.3389/fmars.2021.677679
M3 - Article
AN - SCOPUS:85118234624
SN - 2296-7745
VL - 8
JO - Frontiers in Marine Science
JF - Frontiers in Marine Science
M1 - 677679
ER -