Lessons on enzyme kinetics from quantitative proteomics

Dan Davidi, Ron Milo

Research output: Contribution to journalReview articlepeer-review

35 Scopus citations

Abstract

Enzyme kinetics are fundamental to an understanding of cellular metabolism and for crafting synthetic biology applications. For decades, enzyme characterization has been based on in vitro enzyme assays. However, kinetic parameters are only available for <10% of reactions, and this data scarcity limits the predictive power of metabolic models. Here we review recent studies that leverage quantitative proteomics to gain insight into in vivo enzyme kinetics. We discuss findings on the relationship between in vivo and in vitro enzyme catalysis and show how proteomics can be used to characterize the efficiency of enzyme utilization across conditions. Lastly, the efficient use of enzymes is shown to rationalize preference for low energy-yield metabolic strategies, such as aerobic fermentation at high growth rate.

Original languageEnglish
Pages (from-to)81-89
Number of pages9
JournalCurrent Opinion in Biotechnology
Volume46
DOIs
StatePublished - 1 Aug 2017
Externally publishedYes

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