TY - JOUR
T1 - Lessons on enzyme kinetics from quantitative proteomics
AU - Davidi, Dan
AU - Milo, Ron
N1 - Funding Information:
The authors thank Elad Noor, Arren Bar-Even, Uri Barenholz, Avi Flamholz, Yinon Bar-On, Wolfram Liebermeister, Nathan Lewis, Karl Kochanowski, Noam Prywes, David Wernick, Alexander Geller and Lianet Noda for productive discussions and critical reading of the manuscript. This research was funded by the European Research Council (Project NOVCARBFIX 646827); the Israel Science Foundation (grant no. 740/16); Beck-Canadian Center for Alternative Energy Research; Dana and Yossie Hollander; Helmsley Charitable Foundation; The Larson Charitable Foundation; Wolfson Family Charitable Trust; Charles Rothschild; Selmo Nussenbaum. R.M. is the Charles and Louise Gartner professional chair.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Enzyme kinetics are fundamental to an understanding of cellular metabolism and for crafting synthetic biology applications. For decades, enzyme characterization has been based on in vitro enzyme assays. However, kinetic parameters are only available for <10% of reactions, and this data scarcity limits the predictive power of metabolic models. Here we review recent studies that leverage quantitative proteomics to gain insight into in vivo enzyme kinetics. We discuss findings on the relationship between in vivo and in vitro enzyme catalysis and show how proteomics can be used to characterize the efficiency of enzyme utilization across conditions. Lastly, the efficient use of enzymes is shown to rationalize preference for low energy-yield metabolic strategies, such as aerobic fermentation at high growth rate.
AB - Enzyme kinetics are fundamental to an understanding of cellular metabolism and for crafting synthetic biology applications. For decades, enzyme characterization has been based on in vitro enzyme assays. However, kinetic parameters are only available for <10% of reactions, and this data scarcity limits the predictive power of metabolic models. Here we review recent studies that leverage quantitative proteomics to gain insight into in vivo enzyme kinetics. We discuss findings on the relationship between in vivo and in vitro enzyme catalysis and show how proteomics can be used to characterize the efficiency of enzyme utilization across conditions. Lastly, the efficient use of enzymes is shown to rationalize preference for low energy-yield metabolic strategies, such as aerobic fermentation at high growth rate.
UR - http://www.scopus.com/inward/record.url?scp=85014874443&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2017.02.007
DO - 10.1016/j.copbio.2017.02.007
M3 - Review article
C2 - 28288339
AN - SCOPUS:85014874443
VL - 46
SP - 81
EP - 89
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
SN - 0958-1669
ER -