Leukotriene C4 decreases the activity of respiratory cilia in vitro

Z. WEISMAN; A. FINK; A. ALON; Z. POLIAK; E. TABACHNIK; L. PRISCU; Z. BENTWICH

doi:10.1111/j.1365-2222.1990.tb02799.x

Leukotriene C₄ decreases the activity of respiratory cilia in vitro

Z. WEISMAN, A. FINK, A. ALON, Z. POLIAK, E. TABACHNIK, L. PRISCU, Z. BENTWICH

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

In the present study we assessed the effect of leukotriene C₄ on ciliary beat frequency in vitro. Chicken tracheas were sliced into thin rings and ciliary activity was viewed microscopically. Ciliary beat frequency was measured through an optical fibre by a fast Fourier transformer analyser and recorded on an oscilloscope. Ciliary beat frequency was measured at one fixed point during 30 min at room temperature in RPMI‐HEPES medium alone and in medium containing leukotriene C₄ at a range of concentrations (10⁻⁶–10⁻⁹M). Our results demonstrate that leukotriene C₄(10⁻⁷ and 10⁻⁸M) caused a significant decrease in ciliary beat frequency in all tracheal rings tested (mean decrease of 24%). The specificity and Ca²⁺ ‐dependency of leukotriene C₄ activity was elucidated by the ability of FPL 55712 (SRS‐A specific antagonist), Ca²⁺ ‐free medium, calcium channel blockers (nifedipine and verapamil) and the calmodulin inhibitor trifluroperazine to abrogate its effect on ciliary beating.

Original language	English
Pages (from-to)	389-393
Number of pages	5
Journal	Clinical and Experimental Allergy
Volume	20
Issue number	4
DOIs	https://doi.org/10.1111/j.1365-2222.1990.tb02799.x
State	Published - 1 Jan 1990
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1111/j.1365-2222.1990.tb02799.x

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title = "Leukotriene C4 decreases the activity of respiratory cilia in vitro",

abstract = "In the present study we assessed the effect of leukotriene C4 on ciliary beat frequency in vitro. Chicken tracheas were sliced into thin rings and ciliary activity was viewed microscopically. Ciliary beat frequency was measured through an optical fibre by a fast Fourier transformer analyser and recorded on an oscilloscope. Ciliary beat frequency was measured at one fixed point during 30 min at room temperature in RPMI‐HEPES medium alone and in medium containing leukotriene C4 at a range of concentrations (10−6–10−9M). Our results demonstrate that leukotriene C4(10−7 and 10−8M) caused a significant decrease in ciliary beat frequency in all tracheal rings tested (mean decrease of 24%). The specificity and Ca2+ ‐dependency of leukotriene C4 activity was elucidated by the ability of FPL 55712 (SRS‐A specific antagonist), Ca2+ ‐free medium, calcium channel blockers (nifedipine and verapamil) and the calmodulin inhibitor trifluroperazine to abrogate its effect on ciliary beating.",

author = "Z. WEISMAN and A. FINK and A. ALON and Z. POLIAK and E. TABACHNIK and L. PRISCU and Z. BENTWICH",

year = "1990",

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doi = "10.1111/j.1365-2222.1990.tb02799.x",

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AU - WEISMAN, Z.

AU - FINK, A.

AU - ALON, A.

AU - POLIAK, Z.

AU - TABACHNIK, E.

AU - PRISCU, L.

AU - BENTWICH, Z.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - In the present study we assessed the effect of leukotriene C4 on ciliary beat frequency in vitro. Chicken tracheas were sliced into thin rings and ciliary activity was viewed microscopically. Ciliary beat frequency was measured through an optical fibre by a fast Fourier transformer analyser and recorded on an oscilloscope. Ciliary beat frequency was measured at one fixed point during 30 min at room temperature in RPMI‐HEPES medium alone and in medium containing leukotriene C4 at a range of concentrations (10−6–10−9M). Our results demonstrate that leukotriene C4(10−7 and 10−8M) caused a significant decrease in ciliary beat frequency in all tracheal rings tested (mean decrease of 24%). The specificity and Ca2+ ‐dependency of leukotriene C4 activity was elucidated by the ability of FPL 55712 (SRS‐A specific antagonist), Ca2+ ‐free medium, calcium channel blockers (nifedipine and verapamil) and the calmodulin inhibitor trifluroperazine to abrogate its effect on ciliary beating.

AB - In the present study we assessed the effect of leukotriene C4 on ciliary beat frequency in vitro. Chicken tracheas were sliced into thin rings and ciliary activity was viewed microscopically. Ciliary beat frequency was measured through an optical fibre by a fast Fourier transformer analyser and recorded on an oscilloscope. Ciliary beat frequency was measured at one fixed point during 30 min at room temperature in RPMI‐HEPES medium alone and in medium containing leukotriene C4 at a range of concentrations (10−6–10−9M). Our results demonstrate that leukotriene C4(10−7 and 10−8M) caused a significant decrease in ciliary beat frequency in all tracheal rings tested (mean decrease of 24%). The specificity and Ca2+ ‐dependency of leukotriene C4 activity was elucidated by the ability of FPL 55712 (SRS‐A specific antagonist), Ca2+ ‐free medium, calcium channel blockers (nifedipine and verapamil) and the calmodulin inhibitor trifluroperazine to abrogate its effect on ciliary beating.

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JO - Clinical and Experimental Allergy

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Leukotriene C₄ decreases the activity of respiratory cilia in vitro

Abstract

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