Lipid/polydiacetylene films for colorimetric protein surface-charge analysis

Sarit Friedman, Sofiya Kolusheva, Roman Volinsky, Leila Zeiri, Thomas Schrader, Raz Jelinek

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The distribution and organization of charges on a protein surface are fundamental properties which affect protein functions and interactions. We demonstrate a new approach for protein surface-charge analysis through modulating protein interactions with chromatic lipid/polydiacetylene (PDA) films. We show that visible and easily quantifiable blue-red transitions, induced on the film surface through electrostatic interactions between the negatively charged PDA and positive soluble species, constitute an effective means for characterizing protein surface charge. Specifically, protein-film interactions can be significantly modulated by complexation between the tested macromolecules and lipid-embedded multivalent calixarene ligands displaying charged residues, making possible protein discrimination based upon the abundance and organization of surface charge. The lipid/PDA film system, in conjunction with the calixarene-derived ligands, facilitates characterization of protein surface charges and identification of anomalous protein electrostatic properties.

Original languageEnglish
Pages (from-to)7804-7811
Number of pages8
JournalAnalytical Chemistry
Volume80
Issue number20
DOIs
StatePublished - 15 Oct 2008

ASJC Scopus subject areas

  • Analytical Chemistry

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