TY - JOUR
T1 - Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells
T2 - A boron neutron capture therapy study
AU - Gifford, Ian
AU - Vreeland, Wyatt
AU - Grdanovska, Slavica
AU - Burgett, Eric
AU - Kalinich, John
AU - Vergara, Vernieda
AU - Wang, C. K.Chris
AU - Maimon, Eric
AU - Poster, Dianne
AU - Al-Sheikhly, Mohamad
N1 - Funding Information:
1Armed Forces Radiobiology Research Institute, Bethesda, MD, 2National Institute of Standards and Technology, Gaithersburg, MD, 3 Graduate Program in Nuclear Engineering and 7Department of Materials Science and Engineering, University of Maryland, College Park, MD, 4 Department of Nuclear Engineering and Health Physics, Idaho State University, Pocatello, ID, 5Nuclear Engineering and Medical Physics Program, Georgia Institute of Technology, Atlanta, GA, USA, and 6 Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva , Israel
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Purpose: The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. Materials and methods: Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometry (ICP-MS) and high-performance liquid chromatography (HPLC). Cytotoxicity of the BCH-containing liposomes was evaluated with neutral red, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS), and lactate dehydrogenase assays. Colony formation assays were utilized to evaluate the decrease in cell survival due to high-linear energy transfer (LET) particles resulting from 10B thermal neutron capture. Results: BCH delivery by means of encapsulation in a lipid bilayer resulted in a boron uptake of 35.2 ± 4.3 μg/109 cells, with minimal cytotoxic effects. PC-3 cells treated with BCH and exposed to a 9.4 × 1011 n/cm2 thermal neutron fluence yielded a 20-25% decrease in clonogenic capacity. The decreased survival is attributed to the generation of high-LET α particles and 7Li nuclei that deposit energy in densely ionizing radiation tracks. Conclusion: Liposome-based delivery of BCH is capable of introducing sufficient boron to PC-3 cells for BNCT. High-LET α particles and 7Li nuclei generated from 10B thermal neutron capture significantly decrease colony formation ability in the targeted PC-3 cells.
AB - Purpose: The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. Materials and methods: Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometry (ICP-MS) and high-performance liquid chromatography (HPLC). Cytotoxicity of the BCH-containing liposomes was evaluated with neutral red, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS), and lactate dehydrogenase assays. Colony formation assays were utilized to evaluate the decrease in cell survival due to high-linear energy transfer (LET) particles resulting from 10B thermal neutron capture. Results: BCH delivery by means of encapsulation in a lipid bilayer resulted in a boron uptake of 35.2 ± 4.3 μg/109 cells, with minimal cytotoxic effects. PC-3 cells treated with BCH and exposed to a 9.4 × 1011 n/cm2 thermal neutron fluence yielded a 20-25% decrease in clonogenic capacity. The decreased survival is attributed to the generation of high-LET α particles and 7Li nuclei that deposit energy in densely ionizing radiation tracks. Conclusion: Liposome-based delivery of BCH is capable of introducing sufficient boron to PC-3 cells for BNCT. High-LET α particles and 7Li nuclei generated from 10B thermal neutron capture significantly decrease colony formation ability in the targeted PC-3 cells.
KW - BNCT
KW - Boron neutron capture therapy
KW - Prostate cancer cells
KW - Thermal neutron irradiation
UR - http://www.scopus.com/inward/record.url?scp=84901921125&partnerID=8YFLogxK
U2 - 10.3109/09553002.2014.901579
DO - 10.3109/09553002.2014.901579
M3 - Article
C2 - 24605770
AN - SCOPUS:84901921125
SN - 0955-3002
VL - 90
SP - 480
EP - 485
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 6
ER -