Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study

Ian Gifford; Wyatt Vreeland; Slavica Grdanovska; Eric Burgett; John Kalinich; Vernieda Vergara; C. K.Chris Wang; Eric Maimon; Dianne Poster; Mohamad Al-Sheikhly

doi:10.3109/09553002.2014.901579

Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study

Ian Gifford
, Wyatt Vreeland
, Slavica Grdanovska
, Eric Burgett
, John Kalinich
, Vernieda Vergara
, C. K.Chris Wang
, Eric Maimon
, Dianne Poster
, Mohamad Al-Sheikhly

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Purpose: The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. Materials and methods: Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometry (ICP-MS) and high-performance liquid chromatography (HPLC). Cytotoxicity of the BCH-containing liposomes was evaluated with neutral red, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS), and lactate dehydrogenase assays. Colony formation assays were utilized to evaluate the decrease in cell survival due to high-linear energy transfer (LET) particles resulting from ¹⁰B thermal neutron capture. Results: BCH delivery by means of encapsulation in a lipid bilayer resulted in a boron uptake of 35.2 ± 4.3 μg/10⁹ cells, with minimal cytotoxic effects. PC-3 cells treated with BCH and exposed to a 9.4 × 10¹¹ n/cm² thermal neutron fluence yielded a 20-25% decrease in clonogenic capacity. The decreased survival is attributed to the generation of high-LET α particles and ⁷Li nuclei that deposit energy in densely ionizing radiation tracks. Conclusion: Liposome-based delivery of BCH is capable of introducing sufficient boron to PC-3 cells for BNCT. High-LET α particles and ⁷Li nuclei generated from ¹⁰B thermal neutron capture significantly decrease colony formation ability in the targeted PC-3 cells.

Original language	English
Pages (from-to)	480-485
Number of pages	6
Journal	International Journal of Radiation Biology
Volume	90
Issue number	6
DOIs	https://doi.org/10.3109/09553002.2014.901579
State	Published - 1 Jan 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

BNCT
Boron neutron capture therapy
Prostate cancer cells
Thermal neutron irradiation

ASJC Scopus subject areas

Radiological and Ultrasound Technology
Radiology Nuclear Medicine and imaging

Access to Document

10.3109/09553002.2014.901579

Cite this

Gifford, I., Vreeland, W., Grdanovska, S., Burgett, E., Kalinich, J., Vergara, V., Wang, C. K. C., Maimon, E., Poster, D., & Al-Sheikhly, M. (2014). Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study. International Journal of Radiation Biology, 90(6), 480-485. https://doi.org/10.3109/09553002.2014.901579

@article{c3ae37579cb0419798847554a6df7174,

title = "Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study",

abstract = "Purpose: The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. Materials and methods: Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometry (ICP-MS) and high-performance liquid chromatography (HPLC). Cytotoxicity of the BCH-containing liposomes was evaluated with neutral red, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS), and lactate dehydrogenase assays. Colony formation assays were utilized to evaluate the decrease in cell survival due to high-linear energy transfer (LET) particles resulting from 10B thermal neutron capture. Results: BCH delivery by means of encapsulation in a lipid bilayer resulted in a boron uptake of 35.2 ± 4.3 μg/109 cells, with minimal cytotoxic effects. PC-3 cells treated with BCH and exposed to a 9.4 × 1011 n/cm2 thermal neutron fluence yielded a 20-25\% decrease in clonogenic capacity. The decreased survival is attributed to the generation of high-LET α particles and 7Li nuclei that deposit energy in densely ionizing radiation tracks. Conclusion: Liposome-based delivery of BCH is capable of introducing sufficient boron to PC-3 cells for BNCT. High-LET α particles and 7Li nuclei generated from 10B thermal neutron capture significantly decrease colony formation ability in the targeted PC-3 cells.",

keywords = "BNCT, Boron neutron capture therapy, Prostate cancer cells, Thermal neutron irradiation",

author = "Ian Gifford and Wyatt Vreeland and Slavica Grdanovska and Eric Burgett and John Kalinich and Vernieda Vergara and Wang, \{C. K.Chris\} and Eric Maimon and Dianne Poster and Mohamad Al-Sheikhly",

note = "Funding Information: 1Armed Forces Radiobiology Research Institute, Bethesda, MD, 2National Institute of Standards and Technology, Gaithersburg, MD, 3 Graduate Program in Nuclear Engineering and 7Department of Materials Science and Engineering, University of Maryland, College Park, MD, 4 Department of Nuclear Engineering and Health Physics, Idaho State University, Pocatello, ID, 5Nuclear Engineering and Medical Physics Program, Georgia Institute of Technology, Atlanta, GA, USA, and 6 Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva , Israel",

year = "2014",

month = jan,

day = "1",

doi = "10.3109/09553002.2014.901579",

language = "English",

volume = "90",

pages = "480--485",

journal = "International Journal of Radiation Biology",

issn = "0955-3002",

publisher = "Taylor and Francis Ltd.",

number = "6",

}

Gifford, I, Vreeland, W, Grdanovska, S, Burgett, E, Kalinich, J, Vergara, V, Wang, CKC, Maimon, E, Poster, D & Al-Sheikhly, M 2014, 'Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study', International Journal of Radiation Biology, vol. 90, no. 6, pp. 480-485. https://doi.org/10.3109/09553002.2014.901579

Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study. / Gifford, Ian; Vreeland, Wyatt; Grdanovska, Slavica et al.
In: International Journal of Radiation Biology, Vol. 90, No. 6, 01.01.2014, p. 480-485.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells

T2 - A boron neutron capture therapy study

AU - Gifford, Ian

AU - Vreeland, Wyatt

AU - Grdanovska, Slavica

AU - Burgett, Eric

AU - Kalinich, John

AU - Vergara, Vernieda

AU - Wang, C. K.Chris

AU - Maimon, Eric

AU - Poster, Dianne

AU - Al-Sheikhly, Mohamad

N1 - Funding Information: 1Armed Forces Radiobiology Research Institute, Bethesda, MD, 2National Institute of Standards and Technology, Gaithersburg, MD, 3 Graduate Program in Nuclear Engineering and 7Department of Materials Science and Engineering, University of Maryland, College Park, MD, 4 Department of Nuclear Engineering and Health Physics, Idaho State University, Pocatello, ID, 5Nuclear Engineering and Medical Physics Program, Georgia Institute of Technology, Atlanta, GA, USA, and 6 Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva , Israel

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Purpose: The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. Materials and methods: Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometry (ICP-MS) and high-performance liquid chromatography (HPLC). Cytotoxicity of the BCH-containing liposomes was evaluated with neutral red, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS), and lactate dehydrogenase assays. Colony formation assays were utilized to evaluate the decrease in cell survival due to high-linear energy transfer (LET) particles resulting from 10B thermal neutron capture. Results: BCH delivery by means of encapsulation in a lipid bilayer resulted in a boron uptake of 35.2 ± 4.3 μg/109 cells, with minimal cytotoxic effects. PC-3 cells treated with BCH and exposed to a 9.4 × 1011 n/cm2 thermal neutron fluence yielded a 20-25% decrease in clonogenic capacity. The decreased survival is attributed to the generation of high-LET α particles and 7Li nuclei that deposit energy in densely ionizing radiation tracks. Conclusion: Liposome-based delivery of BCH is capable of introducing sufficient boron to PC-3 cells for BNCT. High-LET α particles and 7Li nuclei generated from 10B thermal neutron capture significantly decrease colony formation ability in the targeted PC-3 cells.

AB - Purpose: The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. Materials and methods: Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometry (ICP-MS) and high-performance liquid chromatography (HPLC). Cytotoxicity of the BCH-containing liposomes was evaluated with neutral red, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS), and lactate dehydrogenase assays. Colony formation assays were utilized to evaluate the decrease in cell survival due to high-linear energy transfer (LET) particles resulting from 10B thermal neutron capture. Results: BCH delivery by means of encapsulation in a lipid bilayer resulted in a boron uptake of 35.2 ± 4.3 μg/109 cells, with minimal cytotoxic effects. PC-3 cells treated with BCH and exposed to a 9.4 × 1011 n/cm2 thermal neutron fluence yielded a 20-25% decrease in clonogenic capacity. The decreased survival is attributed to the generation of high-LET α particles and 7Li nuclei that deposit energy in densely ionizing radiation tracks. Conclusion: Liposome-based delivery of BCH is capable of introducing sufficient boron to PC-3 cells for BNCT. High-LET α particles and 7Li nuclei generated from 10B thermal neutron capture significantly decrease colony formation ability in the targeted PC-3 cells.

KW - BNCT

KW - Boron neutron capture therapy

KW - Prostate cancer cells

KW - Thermal neutron irradiation

UR - https://www.scopus.com/pages/publications/84901921125

U2 - 10.3109/09553002.2014.901579

DO - 10.3109/09553002.2014.901579

M3 - Article

C2 - 24605770

AN - SCOPUS:84901921125

SN - 0955-3002

VL - 90

SP - 480

EP - 485

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

IS - 6

ER -

Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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