Live cell labeling of native intracellular bacterial receptors using aniline-catalyzed oxime ligation

Josep Rayo, Neri Amara, Pnina Krief, Michael M. Meijler

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Live cell fluorescent labeling of proteins has become a seminal tool in biology and has led to hallmark discoveries in diverse research areas such as protein trafficking, cell-to-cell interactions, and intracellular network dynamics. One of the main challenges, however, remains the ability to label intracellular proteins using fluorescent ligands with high specificity, all the while retaining viability of the targeted cells. Here, we present the first example of live cell labeling and imaging of an intracellular bacterial receptor involved in cell-to-cell communication (i.e., quorum sensing), using a novel two-step approach involving covalent attachment of a reactive mimic of the primary endogenous Pseudomonas aeruginosa quorum-sensing signal to its receptor, LasR, followed by aniline-catalyzed oxime formation between the modified receptor and a fluorescent BODIPY derivative. Our results indicate that LasR is not distributed evenly throughout the cytoplasmic membrane but is instead concentrated at the poles of the P. aeruginosa cell.

Original languageEnglish
Pages (from-to)7469-7475
Number of pages7
JournalJournal of the American Chemical Society
Volume133
Issue number19
DOIs
StatePublished - 18 May 2011

Fingerprint

Dive into the research topics of 'Live cell labeling of native intracellular bacterial receptors using aniline-catalyzed oxime ligation'. Together they form a unique fingerprint.

Cite this