Live cell poration by au nanostars to probe intracellular molecular composition with SERS

Evelina I. Nikelshparg; Ekaterina S. Prikhozhdenko; Roman A. Verkhovskii; Vsevolod S. Atkin; Vitaly A. Khanadeev; Boris N. Khlebtsov; Daniil N. Bratashov

doi:10.3390/nano11102588

Live cell poration by au nanostars to probe intracellular molecular composition with SERS

Evelina I. Nikelshparg, Ekaterina S. Prikhozhdenko, Roman A. Verkhovskii, Vsevolod S. Atkin, Vitaly A. Khanadeev, Boris N. Khlebtsov, Daniil N. Bratashov

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer of poly (4-vinyl pyridine) polymer. This type of SERS substrate provides good cell adhesion and viability. Au nanostars’ long tips can penetrate the cell membrane, allowing it to receive the SERS signal from biomolecules inside a living cell. The proposed nanostructured surfaces were tested to study, label-free, the distribution of various biomolecules in cell compartments.

Original language	English
Article number	2588
Journal	Nanomaterials
Volume	11
Issue number	10
DOIs	https://doi.org/10.3390/nano11102588
State	Published - 1 Oct 2021
Externally published	Yes

Keywords

Au nanostars
Cell membrane
SERS

ASJC Scopus subject areas

General Chemical Engineering
General Materials Science

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10.3390/nano11102588

Cite this

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title = "Live cell poration by au nanostars to probe intracellular molecular composition with SERS",

abstract = "A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer of poly (4-vinyl pyridine) polymer. This type of SERS substrate provides good cell adhesion and viability. Au nanostars{\textquoteright} long tips can penetrate the cell membrane, allowing it to receive the SERS signal from biomolecules inside a living cell. The proposed nanostructured surfaces were tested to study, label-free, the distribution of various biomolecules in cell compartments.",

keywords = "Au nanostars, Cell membrane, SERS",

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doi = "10.3390/nano11102588",

language = "English",

volume = "11",

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AU - Nikelshparg, Evelina I.

AU - Prikhozhdenko, Ekaterina S.

AU - Verkhovskii, Roman A.

AU - Atkin, Vsevolod S.

AU - Khanadeev, Vitaly A.

AU - Khlebtsov, Boris N.

AU - Bratashov, Daniil N.

PY - 2021/10/1

Y1 - 2021/10/1

N2 - A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer of poly (4-vinyl pyridine) polymer. This type of SERS substrate provides good cell adhesion and viability. Au nanostars’ long tips can penetrate the cell membrane, allowing it to receive the SERS signal from biomolecules inside a living cell. The proposed nanostructured surfaces were tested to study, label-free, the distribution of various biomolecules in cell compartments.

AB - A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer of poly (4-vinyl pyridine) polymer. This type of SERS substrate provides good cell adhesion and viability. Au nanostars’ long tips can penetrate the cell membrane, allowing it to receive the SERS signal from biomolecules inside a living cell. The proposed nanostructured surfaces were tested to study, label-free, the distribution of various biomolecules in cell compartments.

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KW - Cell membrane

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JO - Nanomaterials

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Live cell poration by au nanostars to probe intracellular molecular composition with SERS

Abstract

Keywords

ASJC Scopus subject areas

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