TY - JOUR
T1 - Localization, quantitation, and in situ detection of specific peptide- MHC class I complexes using a monoclonal antibody
AU - Porgador, Angel
AU - Yewdell, Jonathan W.
AU - Deng, Yuping
AU - Bennink, Jack R.
AU - Germain, Ronald N.
N1 - Funding Information:
Correspondence should be addressed to R. N. G. (e-mail: [email protected]). We are grateful to Dr. D. H. Margulies for providing the VSV, Sendai, and C7 peptides, as well as the Y3 antibody, and to Dr. J. Sheil for providing L-K b cells. We also thank Dr. Margulies and Dr. C. Reis e Sousa for helpful comments on the manuscript. We thank Ms. A. Fox for technical assistance. A. P. is a recipient of a Sara Belk Gambrell fellowship from the Cancer Research Institute (New York, New York).
PY - 1997/1/1
Y1 - 1997/1/1
N2 - CD8+ T lymphocytes recognize antigens as short peptides bound to MHC class I molecules. Available methods cannot determine the number and distribution of these ligands on individual cells or detect antigen- presenting cells in tissues. Here we describe a method for eliciting and identifying monoclonal antibodies specific for a particular peptide-MHC class I combination. One such antibody can identify antigen complexes with a limit of detection approaching that of T cells. We used this antibody to determine the number of peptide-class I complexes generated upon viral infection, to identify antigen-presenting cells in cell mixtures, to determine the site of peptide-MHC class I interaction inside cells, and to visualize cells bearing specific peptide-MHC class I complexes after in vivo infection. Similar antibodies may prove useful for diagnostic or therapeutic purposes in cancer, infectious diseases, and autoimmune disorders.
AB - CD8+ T lymphocytes recognize antigens as short peptides bound to MHC class I molecules. Available methods cannot determine the number and distribution of these ligands on individual cells or detect antigen- presenting cells in tissues. Here we describe a method for eliciting and identifying monoclonal antibodies specific for a particular peptide-MHC class I combination. One such antibody can identify antigen complexes with a limit of detection approaching that of T cells. We used this antibody to determine the number of peptide-class I complexes generated upon viral infection, to identify antigen-presenting cells in cell mixtures, to determine the site of peptide-MHC class I interaction inside cells, and to visualize cells bearing specific peptide-MHC class I complexes after in vivo infection. Similar antibodies may prove useful for diagnostic or therapeutic purposes in cancer, infectious diseases, and autoimmune disorders.
UR - http://www.scopus.com/inward/record.url?scp=0030849769&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80447-1
DO - 10.1016/S1074-7613(00)80447-1
M3 - Article
AN - SCOPUS:0030849769
SN - 1074-7613
VL - 6
SP - 715
EP - 726
JO - Immunity
JF - Immunity
IS - 6
ER -
