Localization, quantitation, and in situ detection of specific peptide- MHC class I complexes using a monoclonal antibody

Angel Porgador, Jonathan W. Yewdell, Yuping Deng, Jack R. Bennink, Ronald N. Germain

Research output: Contribution to journalArticlepeer-review

576 Scopus citations

Abstract

CD8+ T lymphocytes recognize antigens as short peptides bound to MHC class I molecules. Available methods cannot determine the number and distribution of these ligands on individual cells or detect antigen- presenting cells in tissues. Here we describe a method for eliciting and identifying monoclonal antibodies specific for a particular peptide-MHC class I combination. One such antibody can identify antigen complexes with a limit of detection approaching that of T cells. We used this antibody to determine the number of peptide-class I complexes generated upon viral infection, to identify antigen-presenting cells in cell mixtures, to determine the site of peptide-MHC class I interaction inside cells, and to visualize cells bearing specific peptide-MHC class I complexes after in vivo infection. Similar antibodies may prove useful for diagnostic or therapeutic purposes in cancer, infectious diseases, and autoimmune disorders.

Original languageEnglish
Pages (from-to)715-726
JournalImmunity
Volume6
Issue number6
DOIs
StatePublished - 1 Jan 1997
Externally publishedYes

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