Localizing cell division in spherical Escherichia coli by nucleoid occlusion

Arieh Zaritsky, Conrad L. Woldringh

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The spatial relationship between FtsZ localization and nucleoid segregation was followed in Escherichia coli thyA cells, made spheroidal by brief exposure to mecillinam and after manipulating chromosome replication time using changes ('steps') in thymine concentration [Zaritsky et al., Microbiology 145 (1999) 1015-1022]. In such cells, fluorescent FtsZ-GFP arcs did not overlap the DAPI-stained nucleoids. It is concluded that FtsZ rings are deposited between segregating nucleoids, consistent with the nucleoid occlusion model [Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038].

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalFEMS Microbiology Letters
Issue number2
StatePublished - 26 Sep 2003


  • Bacterial nucleoid
  • FtsZ-GFP and DAPI fluorescence
  • Spatial control of cell division

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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