Abstract
The spatial relationship between FtsZ localization and nucleoid segregation was followed in Escherichia coli thyA cells, made spheroidal by brief exposure to mecillinam and after manipulating chromosome replication time using changes ('steps') in thymine concentration [Zaritsky et al., Microbiology 145 (1999) 1015-1022]. In such cells, fluorescent FtsZ-GFP arcs did not overlap the DAPI-stained nucleoids. It is concluded that FtsZ rings are deposited between segregating nucleoids, consistent with the nucleoid occlusion model [Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038].
| Original language | English |
|---|---|
| Pages (from-to) | 209-214 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 226 |
| Issue number | 2 |
| DOIs | |
| State | Published - 26 Sep 2003 |
Keywords
- Bacterial nucleoid
- FtsZ-GFP and DAPI fluorescence
- Spatial control of cell division
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics