Localizing cell division in spherical Escherichia coli by nucleoid occlusion

Arieh Zaritsky, Conrad L. Woldringh

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The spatial relationship between FtsZ localization and nucleoid segregation was followed in Escherichia coli thyA cells, made spheroidal by brief exposure to mecillinam and after manipulating chromosome replication time using changes ('steps') in thymine concentration [Zaritsky et al., Microbiology 145 (1999) 1015-1022]. In such cells, fluorescent FtsZ-GFP arcs did not overlap the DAPI-stained nucleoids. It is concluded that FtsZ rings are deposited between segregating nucleoids, consistent with the nucleoid occlusion model [Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038].

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalFEMS Microbiology Letters
Volume226
Issue number2
DOIs
StatePublished - 26 Sep 2003

Keywords

  • Bacterial nucleoid
  • FtsZ-GFP and DAPI fluorescence
  • Spatial control of cell division

Fingerprint

Dive into the research topics of 'Localizing cell division in spherical Escherichia coli by nucleoid occlusion'. Together they form a unique fingerprint.

Cite this