TY - JOUR
T1 - Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds
AU - Ben-Dov, Eitan
AU - Kushmaro, Ariel
AU - Brenner, Asher
N1 - Funding Information:
This project was supported by a grant from the Ramat-Hovav Industrial Council, Israel and BMBF-MOST Cooperation in Water Technologies Grant WT-501. Special thanks are conveyed to the management and staff of the Ramat-Hovav Council for their cooperation. The authors thank Lar- issa Shemtov, Lisa Arkhangelsky, Ronnen Veitsman, Nachshon Siboni and Orr Shapiro, for technical support and useful comments on the manuscript.
PY - 2009/3/19
Y1 - 2009/3/19
N2 - Abundance and seasonal dynamics of sulfate-reducing bacteria (SRB), in general, and of extreme halophilic SRB (belonging to Desulfocella halophila) in particular, were examined in highly saline industrial wastewater evaporation ponds over a forty one month period. Industrial wastewater was sampled and the presence of SRB was determined by quantitative real-time PCR (qPCR) with a set of primers designed to amplify the dissimilatory sulfite reductase (dsrA) gene. SRB displayed higher abundance during the summer (106-10 8targets ml-1) and lower abundance from the autumn-spring (103-105targets ml-1). However, addition of concentrated dissolved organic matter into the evaporation ponds during winter immediately resulted in a proliferation of SRB, despite the lower wastewater temperature (12-14°C). These results indicate that the qPCR approach can be used for rapid measurement of SRB to provide valuable information about the abundance of SRB in harsh environments, such as highly saline industrial wastewaters. Low level of H2S has been maintained over five years, which indicates a possible inhibition of SRB activity, following artificial salination (≈16% w/v of NaCl) of wastewater evaporation ponds, despite SRB reproduction being detected by qPCR.
AB - Abundance and seasonal dynamics of sulfate-reducing bacteria (SRB), in general, and of extreme halophilic SRB (belonging to Desulfocella halophila) in particular, were examined in highly saline industrial wastewater evaporation ponds over a forty one month period. Industrial wastewater was sampled and the presence of SRB was determined by quantitative real-time PCR (qPCR) with a set of primers designed to amplify the dissimilatory sulfite reductase (dsrA) gene. SRB displayed higher abundance during the summer (106-10 8targets ml-1) and lower abundance from the autumn-spring (103-105targets ml-1). However, addition of concentrated dissolved organic matter into the evaporation ponds during winter immediately resulted in a proliferation of SRB, despite the lower wastewater temperature (12-14°C). These results indicate that the qPCR approach can be used for rapid measurement of SRB to provide valuable information about the abundance of SRB in harsh environments, such as highly saline industrial wastewaters. Low level of H2S has been maintained over five years, which indicates a possible inhibition of SRB activity, following artificial salination (≈16% w/v of NaCl) of wastewater evaporation ponds, despite SRB reproduction being detected by qPCR.
UR - http://www.scopus.com/inward/record.url?scp=62249149488&partnerID=8YFLogxK
U2 - 10.1186/1746-1448-5-2
DO - 10.1186/1746-1448-5-2
M3 - Article
C2 - 19226456
AN - SCOPUS:62249149488
SN - 1746-1448
VL - 5
JO - Saline Systems
JF - Saline Systems
IS - 1
M1 - 2
ER -
