Low cytotoxicity of inorganic nanotubes and fullerene-like nanostructures in human bronchial epithelial cells: Relation to inflammatory gene induction and antioxidant response

Michal Pardo, Timor Shuster-Meiseles, Smadar Levin-Zaidman, Assaf Rudich, Yinon Rudich

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

The cytotoxicity of tungsten disulfide nano tubes (INT-WS2) and inorganic fullerene-like molybdenum disulfide (IF-MoS2) nanoparticles (NPs) used in industrial and medical applications was evaluated in comparison to standard environmental particulate matter. The IF-MoS2 and INT-WS2 reside in vesicles/inclusion bodies, suggestive of endocytic vesicles. In cells representing the respiratory, immune and metabolic systems, both IF-MoS2 and INT-WS2 NPs remained nontoxic compared to equivalent concentrations (up to 100 μg/mL in the medium) of silica dioxide (SiO2), diesel engine-derived and carbon black NPs, which induced cell death. Associating with this biocompatibility of IF-MoS2\INT- WS2, we demonstrate in nontransformed human bronchial cells (NL-20) relative low induction of the pro-inflammatory cytokines IL-1β, IL-6, IL-8, and TNF-α. Moreover, IF-MoS2 and INT-WS2 activated antioxidant response as measured by the antioxidant response element (ARE) using a luciferase reporter, and induced Nrf2-mediated Phase II detoxification genes. Collectively, our findings suggest that the lower cytotoxicity of IF-MoS 2 and INT-WS2 NPs does not reflect general biological inertness. Rather, compared to other NP's, it likely results from decreased pro-inflammatory activation, but a comparable significant capacity to induce protective antioxidant/detoxification defense mechanisms.

Original languageEnglish
Pages (from-to)3457-3466
Number of pages10
JournalEnvironmental Science & Technology
Volume48
Issue number6
DOIs
StatePublished - 18 Mar 2014

Fingerprint

Dive into the research topics of 'Low cytotoxicity of inorganic nanotubes and fullerene-like nanostructures in human bronchial epithelial cells: Relation to inflammatory gene induction and antioxidant response'. Together they form a unique fingerprint.

Cite this