Low light cmos contact imager with an integrated poly-acrylic emission filter for fluorescence detection

Yonathan Dattner, Orly Yadid-Pecht

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

This study presents the fabrication of a low cost poly-acrylic acid (PAA) based emission filter integrated with a low light CMOS contact imager for fluorescence detection. The process involves the use of PAA as an adhesive for the emission filter. The poly-acrylic solution was chosen due its optical transparent properties, adhesive properties, miscibility with polar protic solvents and most importantly its bio-compatibility with a biological environment. The emission filter, also known as an absorption filter, involves dissolving an absorbing specimen in a polar protic solvent and mixing it with the PAA to uniformly bond the absorbing specimen and harden the filter. The PAA is optically transparent in solid form and therefore does not contribute to the absorbance of light in the visible spectrum. Many combinations of absorbing specimen and polar protic solvents can be derived, yielding different filter characteristics in different parts of the spectrum. We report a specific combination as a first example of implementation of our technology. The filter reported has excitation in the green spectrum and emission in the red spectrum, utilizing the increased quantum efficiency of the photo sensitive sensor array. The thickness of the filter (20 μm) was chosen by calculating the desired SNR using Beer-Lambert's law for liquids, Quantum Yield of the fluorophore and the Quantum Efficiency of the sensor array. The filters promising characteristics make it suitable for low light fluorescence detection. The filter was integrated with a fully functional low noise, low light CMOS contact imager and experimental results using fluorescence polystyrene micro-spheres are presented.

Original languageEnglish
Pages (from-to)5014-5027
Number of pages14
JournalSensors
Volume10
Issue number5
DOIs
StatePublished - 1 May 2010
Externally publishedYes

Keywords

  • CMOS contact imager
  • Polar protic solvents
  • Poly-acrylic acid
  • Quantum efficiency
  • Quantum yield

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