Low-voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells

Moise Bendayan, Diane Gingras, Ehud Ziv, Yosef S. Haviv

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Novel approach in low voltage transmission electron microscopy (TEM) has revealed the presence of SV40 viral like particles in the secretory zymogen granules and in spherical membrane-bound dense bodies of SV40 infected pancreatic cells. The presence of SV40 antigen in these cellular compartments was confirmed by immunocytochemistry of the VP1 antigen. Visualization of the viral particles was only possible by examining ultrathin tissue sections with low-voltage TEM that significantly enhances imaging contrast. Results indicate that following infection of the cell entry and trafficking of the viral particles are present in unique cellular compartments such as ER, dense bodies, and secretory granules.

Original languageEnglish
Pages (from-to)659-662
Number of pages4
JournalMicroscopy Research and Technique
Volume71
Issue number9
DOIs
StatePublished - 1 Sep 2008
Externally publishedYes

Keywords

  • Immunogold
  • Low-voltage electron microscopy
  • Pancreas
  • SV40
  • VP1

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