Low-voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells

Moise Bendayan; Diane Gingras; Ehud Ziv; Yosef S. Haviv

doi:10.1002/jemt.20603

Low-voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells

Moise Bendayan, Diane Gingras, Ehud Ziv, Yosef S. Haviv

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Novel approach in low voltage transmission electron microscopy (TEM) has revealed the presence of SV40 viral like particles in the secretory zymogen granules and in spherical membrane-bound dense bodies of SV40 infected pancreatic cells. The presence of SV40 antigen in these cellular compartments was confirmed by immunocytochemistry of the VP1 antigen. Visualization of the viral particles was only possible by examining ultrathin tissue sections with low-voltage TEM that significantly enhances imaging contrast. Results indicate that following infection of the cell entry and trafficking of the viral particles are present in unique cellular compartments such as ER, dense bodies, and secretory granules.

Original language	English
Pages (from-to)	659-662
Number of pages	4
Journal	Microscopy Research and Technique
Volume	71
Issue number	9
DOIs	https://doi.org/10.1002/jemt.20603
State	Published - 1 Jan 2008
Externally published	Yes

Keywords

Immunogold
Low-voltage electron microscopy
Pancreas
SV40
VP1

ASJC Scopus subject areas

Anatomy
Histology
Instrumentation
Medical Laboratory Technology

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10.1002/jemt.20603

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title = "Low-voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells",

abstract = "Novel approach in low voltage transmission electron microscopy (TEM) has revealed the presence of SV40 viral like particles in the secretory zymogen granules and in spherical membrane-bound dense bodies of SV40 infected pancreatic cells. The presence of SV40 antigen in these cellular compartments was confirmed by immunocytochemistry of the VP1 antigen. Visualization of the viral particles was only possible by examining ultrathin tissue sections with low-voltage TEM that significantly enhances imaging contrast. Results indicate that following infection of the cell entry and trafficking of the viral particles are present in unique cellular compartments such as ER, dense bodies, and secretory granules.",

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AU - Bendayan, Moise

AU - Gingras, Diane

AU - Ziv, Ehud

AU - Haviv, Yosef S.

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Y1 - 2008/1/1

N2 - Novel approach in low voltage transmission electron microscopy (TEM) has revealed the presence of SV40 viral like particles in the secretory zymogen granules and in spherical membrane-bound dense bodies of SV40 infected pancreatic cells. The presence of SV40 antigen in these cellular compartments was confirmed by immunocytochemistry of the VP1 antigen. Visualization of the viral particles was only possible by examining ultrathin tissue sections with low-voltage TEM that significantly enhances imaging contrast. Results indicate that following infection of the cell entry and trafficking of the viral particles are present in unique cellular compartments such as ER, dense bodies, and secretory granules.

AB - Novel approach in low voltage transmission electron microscopy (TEM) has revealed the presence of SV40 viral like particles in the secretory zymogen granules and in spherical membrane-bound dense bodies of SV40 infected pancreatic cells. The presence of SV40 antigen in these cellular compartments was confirmed by immunocytochemistry of the VP1 antigen. Visualization of the viral particles was only possible by examining ultrathin tissue sections with low-voltage TEM that significantly enhances imaging contrast. Results indicate that following infection of the cell entry and trafficking of the viral particles are present in unique cellular compartments such as ER, dense bodies, and secretory granules.

KW - Immunogold

KW - Low-voltage electron microscopy

KW - Pancreas

KW - SV40

KW - VP1

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ER -

Low-voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells

Abstract

Keywords

ASJC Scopus subject areas

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