@article{8bd93c5e5ebc41f6b517266226e28214,
title = "Lysine methylation of the NF-κ B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-κ B signaling",
abstract = "Signaling via the methylation of lysine residues in proteins has been linked to diverse biological and disease processes, yet the catalytic activity and substrate specificity of many human protein lysine methyltransferases (PKMTs) are unknown. We screened over 40 candidate PKMTs and identified SETD6 as a methyltransferase that monomethylated chromatin-associated transcription factor NF-κ B subunit RelA at Lys310 (RelAK310me1). SETD6-mediated methylation rendered RelA inert and attenuated RelA-driven transcriptional programs, including inflammatory responses in primary immune cells. RelAK310me1 was recognized by the ankryin repeat of the histone methyltransferase GLP, which under basal conditions promoted a repressed chromatin state at RelA target genes through GLP-mediated methylation of histone H3 Lys9 (H3K9). NF-κ B-activation-linked phosphorylation of RelA at Ser311 by protein kinase C-ω(PKC-ω) blocked the binding of GLP to RelAK310me1 and relieved repression of the target gene. Our findings establish a previously uncharacterized mechanism by which chromatin signaling regulates inflammation programs.",
author = "Dan Levy and Kuo, {Alex J.} and Yanqi Chang and Uwe Schaefer and Christopher Kitson and Peggie Cheung and Alexsandra Espejo and Zee, {Barry M.} and Liu, {Chih Long} and Stephanie Tangsombatvisit and Tennen, {Ruth I.} and Kuo, {Andrew Y.} and Song Tanjing and Regina Cheung and Chua, {Katrin F.} and Utz, {Paul J.} and Xiaobing Shi and Prinjha, {Rab K.} and Kevin Lee and Garcia, {Benjamin A.} and Bedford, {Mark T.} and Alexander Tarakhovsky and Xiaodong Cheng and Or Gozani",
note = "Funding Information: We thank R. Kingston and M. Simon (Harvard Medical School) for recombinant nucleosomes; J. Smith (University of Alabama Birmingham) for the PKCζ(ca) plasmid; W.C. Greene (University of California San Francisco) for RelA(1–431) cDNA and the κBLuc luciferase reporter plasmid; D. Reinberg (New York University) for the NSD1(SET) plasmid; M. Covert (Stanford University) and T.D. Gilmore (Boston University) for the wildtype and Rela−/− mouse 3T3 cells; J. Moscat (University of Cincinnati College of Medicine) for the wildtype and Prkcz−/− MEFs; E. Engleman (Stanford University) for FLB16 cells; E. Green for critical reading of the manuscript; and A. Alizadeh for comments. Supported by the National Institutes of Health (DA025800 to O.G. and M.T.B.; GM068680 to X.C.; and F32AI080086 to C.L.L.), the American Society for Mass Spectrometry (B.A.G.), the National Heart, Lung and Blood Institute (HHSN268201999934C to P.J.U.), the National Institute of Allergy and Infectious Diseases (U19AI082719 to P.J.U.), the Floren Family Trust (P.J.U.), the Genentech Foundation (A.J.K.), the European Molecular Biology Organization (D.L.), the Human Frontier Science Program (D.L.), the Machiah Foundation (D.L.), the Georgia Research Alliance (X.C.) and the Ellison Medical Foundation (O.G.)",
year = "2011",
month = jan,
day = "1",
doi = "10.1038/ni.1968",
language = "English",
volume = "12",
pages = "29--36",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Research",
number = "1",
}