M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

M. P. Danilenko; Z. S. Smagulova; V. K. Turmukhambetova; R. D. Omarova; O. V. Esyrev

M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

Translated title of the contribution: M-cholinergic regulation of Na+,K+-ATPase activity of vesicular preparations of sarcolemma from the myocardium and intestinal smooth muscles

M. P. Danilenko, Z. S. Smagulova, V. K. Turmukhambetova, R. D. Omarova, O. V. Esyrev

Department of Clinical Biochemistry and Pharmacology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.

Translated title of the contribution	M-cholinergic regulation of Na+,K+-ATPase activity of vesicular preparations of sarcolemma from the myocardium and intestinal smooth muscles
Original language	Russian
Pages (from-to)	55-60
Number of pages	6
Journal	Ukrainian Biochemical Journal
Volume	60
Issue number	1
State	Published - 1 Jan 1988

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.",

abstract = "Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.",

author = "Danilenko, {M. P.} and Smagulova, {Z. S.} and Turmukhambetova, {V. K.} and Omarova, {R. D.} and Esyrev, {O. V.}",

year = "1988",

month = jan,

day = "1",

language = "רוסית",

volume = "60",

pages = "55--60",

journal = "Ukrainian Biochemical Journal",

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T1 - M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

AU - Danilenko, M. P.

AU - Smagulova, Z. S.

AU - Turmukhambetova, V. K.

AU - Omarova, R. D.

AU - Esyrev, O. V.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.

AB - Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.

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M3 - מאמר

AN - SCOPUS:0023779693

SN - 0201-8470

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JO - Ukrainian Biochemical Journal

JF - Ukrainian Biochemical Journal

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ER -

M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

Abstract

ASJC Scopus subject areas

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