M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

M. P. Danilenko; Z. S. Smagulova; V. K. Turmukhambetova; R. D. Omarova; O. V. Esyrev

M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

Translated title of the contribution: M-cholinergic regulation of Na+,K+-ATPase activity of vesicular preparations of sarcolemma from the myocardium and intestinal smooth muscles

M. P. Danilenko
, Z. S. Smagulova
, V. K. Turmukhambetova
, R. D. Omarova
, O. V. Esyrev

Department of Clinical Biochemistry and Pharmacology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.

Translated title of the contribution	M-cholinergic regulation of Na+,K+-ATPase activity of vesicular preparations of sarcolemma from the myocardium and intestinal smooth muscles
Original language	Russian
Pages (from-to)	55-60
Number of pages	6
Journal	Ukrainian Biochemical Journal
Volume	60
Issue number	1
State	Published - 1 Jan 1988

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.",

abstract = "Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150\% and was less pronounced (10-20\%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.",

author = "Danilenko, \{M. P.\} and Smagulova, \{Z. S.\} and Turmukhambetova, \{V. K.\} and Omarova, \{R. D.\} and Esyrev, \{O. V.\}",

year = "1988",

month = jan,

day = "1",

language = "רוסית",

volume = "60",

pages = "55--60",

journal = "Ukrainian Biochemical Journal",

issn = "0201-8470",

publisher = "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU)",

number = "1",

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T1 - M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

AU - Danilenko, M. P.

AU - Smagulova, Z. S.

AU - Turmukhambetova, V. K.

AU - Omarova, R. D.

AU - Esyrev, O. V.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.

AB - Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.

UR - https://www.scopus.com/pages/publications/0023779693

M3 - מאמר

AN - SCOPUS:0023779693

SN - 0201-8470

VL - 60

SP - 55

EP - 60

JO - Ukrainian Biochemical Journal

JF - Ukrainian Biochemical Journal

IS - 1

ER -

M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.

Abstract

ASJC Scopus subject areas

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