Magnetically actuated alginate scaffold: A novel platform for promoting tissue organization and vascularization

Yulia Sapir, Emil Ruvinov, Boris Polyak, Smadar Cohen

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

11 Scopus citations

Abstract

Among the greatest hurdles hindering the successful implementation of tissue-engineered cardiac patch as a therapeutic strategy for myocardial repair is the know-how to promote its rapid integration into the host. We previously demonstrated that prevascularization of the engineered cardiac patch improves cardiac repair after myocardial infarction (MI); the mature vessel networks were generated by including affinity-bound angiogenic factors in the patch and its transplantation on the blood vessel-enriched omentum. Here, we describe a novel in vitro strategy to promote the formation of capillary-like networks in cell constructs without supplementing with angiogenic factors. Endothelial cells (ECs) were seeded into macroporous alginate scaffolds impregnated with magnetically responsive nanoparticles (MNPs), and after pre-culture for 24 h under standard conditions the constructs were subjected to an alternating magnetic field of 40 Hz for 7 days. The magnetic stimulation per se promoted EC organization into capillary-like structures with no supplementation of angiogenic factors; in the non-stimulated constructs, the cells formed sheets or aggregates. This chapter describes in detail the preparation method of the MNP-impregnated alginate scaffold, the cultivation setup for the cell construct under magnetic field conditions, and the set of analyses performed to characterize the resultant cell constructs.

Original languageEnglish
Title of host publicationCardiac Tissue Engineering
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages83-95
Number of pages13
ISBN (Print)9781493910465
DOIs
StatePublished - 1 Jan 2014

Publication series

NameMethods in Molecular Biology
Volume1181
ISSN (Print)1064-3745

Keywords

  • Alginate scaffolds
  • Endothelial cells
  • Magnetic alginate composite
  • Magnetic nanoparticles
  • Tissue engineering
  • Vessel-like networks

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