Magnetically targeted endothelial cells for prevention of vascular restenosis

Boris Polyak; Mikhail Medved; Nina Lazareva; Lindsay Steele; Tirth Patel; Ahmad Rai; Menahem Y. Rotenberg; Kimberly Wasko; Andrew R. Kohut; Richard Sensenig; Gennady Friedman

Magnetically targeted endothelial cells for prevention of vascular restenosis

Boris Polyak
, Mikhail Medved
, Nina Lazareva
, Lindsay Steele
, Tirth Patel
, Ahmad Rai
, Menahem Y. Rotenberg
, Kimberly Wasko
, Andrew R. Kohut
, Richard Sensenig
, Gennady Friedman

Department of Biotechnology Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The present study assessed the potential of magnetically mediated delivery of endothelial cells (ECs) to inhibit in-stent stenosis induced by mechanical injury in a rat carotid artery stent angioplasty model. Syngeneic ECs loaded with polymeric magnetic nanoparticles (MNPs) were administered at the distal end of the stented artery using a brief exposure (12 min) to a uniform magnetic field (1.4-kOe). After two months, magnetic localization of ECs demonstrated significant protection from stenosis at the distal part of the stent in the cell therapy group compared to both the proximal part of stent in the cell therapy group and the control (stented, nontreated) group: 1.7-fold (p < 0.001) less reduction in lumen diameter as measured by B-mode and color Doppler ultrasound, 2.3-fold (p < 0.001) less reduction in the ratios of peak systolic velocities as measured by pulsed wave Doppler ultrasound, and 2.1-fold (p < 0.001) attenuation of stenosis as determined through end point morphometric analysis.

Original language	English
Title of host publication	Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017
Editors	Fiona Case, Matthew Laudon, Fiona Case, Bart Romanowicz
Publisher	TechConnect
Pages	67-70
Number of pages	4
ISBN (Electronic)	9780998878201
State	Published - 1 Jan 2017
Event	11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference - Washington, United States Duration: 14 May 2017 → 17 May 2017

Publication series

Name	Advanced Materials - TechConnect Briefs 2017
Volume	3

Conference

Conference	11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference
Country/Territory	United States
City	Washington
Period	14/05/17 → 17/05/17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell therapy
Endothelium
In-stent restenosis
Magnetic targeting
Vascular healing

ASJC Scopus subject areas

Fuel Technology
Surfaces, Coatings and Films
Biotechnology
Fluid Flow and Transfer Processes

Cite this

Polyak, B., Medved, M., Lazareva, N., Steele, L., Patel, T., Rai, A., Rotenberg, M. Y., Wasko, K., Kohut, A. R., Sensenig, R., & Friedman, G. (2017). Magnetically targeted endothelial cells for prevention of vascular restenosis. In F. Case, M. Laudon, F. Case, & B. Romanowicz (Eds.), Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017 (pp. 67-70). (Advanced Materials - TechConnect Briefs 2017; Vol. 3). TechConnect.

@inproceedings{07466c667287452d976e717f57d368cd,

title = "Magnetically targeted endothelial cells for prevention of vascular restenosis",

abstract = "The present study assessed the potential of magnetically mediated delivery of endothelial cells (ECs) to inhibit in-stent stenosis induced by mechanical injury in a rat carotid artery stent angioplasty model. Syngeneic ECs loaded with polymeric magnetic nanoparticles (MNPs) were administered at the distal end of the stented artery using a brief exposure (12 min) to a uniform magnetic field (1.4-kOe). After two months, magnetic localization of ECs demonstrated significant protection from stenosis at the distal part of the stent in the cell therapy group compared to both the proximal part of stent in the cell therapy group and the control (stented, nontreated) group: 1.7-fold (p < 0.001) less reduction in lumen diameter as measured by B-mode and color Doppler ultrasound, 2.3-fold (p < 0.001) less reduction in the ratios of peak systolic velocities as measured by pulsed wave Doppler ultrasound, and 2.1-fold (p < 0.001) attenuation of stenosis as determined through end point morphometric analysis.",

keywords = "Cell therapy, Endothelium, In-stent restenosis, Magnetic targeting, Vascular healing",

author = "Boris Polyak and Mikhail Medved and Nina Lazareva and Lindsay Steele and Tirth Patel and Ahmad Rai and Rotenberg, \{Menahem Y.\} and Kimberly Wasko and Kohut, \{Andrew R.\} and Richard Sensenig and Gennady Friedman",

year = "2017",

month = jan,

day = "1",

language = "English",

series = "Advanced Materials - TechConnect Briefs 2017",

publisher = "TechConnect",

pages = "67--70",

editor = "Fiona Case and Matthew Laudon and Fiona Case and Bart Romanowicz",

booktitle = "Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017",

note = "11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference ; Conference date: 14-05-2017 Through 17-05-2017",

}

Polyak, B, Medved, M, Lazareva, N, Steele, L, Patel, T, Rai, A, Rotenberg, MY, Wasko, K, Kohut, AR, Sensenig, R & Friedman, G 2017, Magnetically targeted endothelial cells for prevention of vascular restenosis. in F Case, M Laudon, F Case & B Romanowicz (eds), Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017. Advanced Materials - TechConnect Briefs 2017, vol. 3, TechConnect, pp. 67-70, 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference, Washington, United States, 14/05/17.

Magnetically targeted endothelial cells for prevention of vascular restenosis. / Polyak, Boris; Medved, Mikhail; Lazareva, Nina et al.
Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017. ed. / Fiona Case; Matthew Laudon; Fiona Case; Bart Romanowicz. TechConnect, 2017. p. 67-70 (Advanced Materials - TechConnect Briefs 2017; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Magnetically targeted endothelial cells for prevention of vascular restenosis

AU - Polyak, Boris

AU - Medved, Mikhail

AU - Lazareva, Nina

AU - Steele, Lindsay

AU - Patel, Tirth

AU - Rai, Ahmad

AU - Rotenberg, Menahem Y.

AU - Wasko, Kimberly

AU - Kohut, Andrew R.

AU - Sensenig, Richard

AU - Friedman, Gennady

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The present study assessed the potential of magnetically mediated delivery of endothelial cells (ECs) to inhibit in-stent stenosis induced by mechanical injury in a rat carotid artery stent angioplasty model. Syngeneic ECs loaded with polymeric magnetic nanoparticles (MNPs) were administered at the distal end of the stented artery using a brief exposure (12 min) to a uniform magnetic field (1.4-kOe). After two months, magnetic localization of ECs demonstrated significant protection from stenosis at the distal part of the stent in the cell therapy group compared to both the proximal part of stent in the cell therapy group and the control (stented, nontreated) group: 1.7-fold (p < 0.001) less reduction in lumen diameter as measured by B-mode and color Doppler ultrasound, 2.3-fold (p < 0.001) less reduction in the ratios of peak systolic velocities as measured by pulsed wave Doppler ultrasound, and 2.1-fold (p < 0.001) attenuation of stenosis as determined through end point morphometric analysis.

AB - The present study assessed the potential of magnetically mediated delivery of endothelial cells (ECs) to inhibit in-stent stenosis induced by mechanical injury in a rat carotid artery stent angioplasty model. Syngeneic ECs loaded with polymeric magnetic nanoparticles (MNPs) were administered at the distal end of the stented artery using a brief exposure (12 min) to a uniform magnetic field (1.4-kOe). After two months, magnetic localization of ECs demonstrated significant protection from stenosis at the distal part of the stent in the cell therapy group compared to both the proximal part of stent in the cell therapy group and the control (stented, nontreated) group: 1.7-fold (p < 0.001) less reduction in lumen diameter as measured by B-mode and color Doppler ultrasound, 2.3-fold (p < 0.001) less reduction in the ratios of peak systolic velocities as measured by pulsed wave Doppler ultrasound, and 2.1-fold (p < 0.001) attenuation of stenosis as determined through end point morphometric analysis.

KW - Cell therapy

KW - Endothelium

KW - In-stent restenosis

KW - Magnetic targeting

KW - Vascular healing

UR - https://www.scopus.com/pages/publications/85029374335

M3 - Conference contribution

AN - SCOPUS:85029374335

T3 - Advanced Materials - TechConnect Briefs 2017

SP - 67

EP - 70

BT - Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017

A2 - Case, Fiona

A2 - Laudon, Matthew

A2 - Case, Fiona

A2 - Romanowicz, Bart

PB - TechConnect

T2 - 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference

Y2 - 14 May 2017 through 17 May 2017

ER -

Magnetically targeted endothelial cells for prevention of vascular restenosis

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

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Cite this