Maintenance of milk protein gene expression in a subpopulation of 7,12-dimethylbenz (a) anthracene-induced rat mammary carcinoma cells grown on attached collagen gels

Mark L. Johnson, Joseph Levy, Jeffrey M. Rosen

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Milk protein gene expression was studied in cell subpopulations of 7,12-dimethylbenz(a)anthracene-induced rat mammary carcinoma cells enriched or depleted for casein production grown on attached collagen gels. Culture of these cells in the presence of 10% fetal bovine serum, insulin (5 μg/ml), hydrocortisone (10 μg/ml), and prolactin (5 μg/ml) maintained α-, β-, and γ-casein and whey acidic protein mRNAs at levels identical to cells isolated from perphenazine-treated rats. Whey acidic protein mRNA levels in the tumor cells relative to the 14-d lactating gland were greater than those of the casein mRNAs. Withdrawal of prolactin from the casein-producing cells resulted in the loss of all four milk protein mRNAs. Subsequent addition of prolactin to the withdrawn cells caused a rapid accumulation of these mRNAs to prewithdrawal levels. Milk protein gene expression in this tumor cell subpopulation is modulated by prolactin (in the presence of insulin and hydrocortisone) in a similar manner to that observed in the normal mammary gland when these tumor cells are cultured on attached collagen gels.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume21
Issue number8
DOIs
StatePublished - 1 Aug 1985
Externally publishedYes

Keywords

  • collagen gels
  • dimethylbenz(a)anthracene tumors
  • milk protein mRNA

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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