Mapping the splicing landscape of the human immune system

Hadas Ner-Gaon, Ronnie Peleg, Roi Gazit, Anat Reiner-Benaim, Tal Shay

Research output: Contribution to journalArticlepeer-review


Most human genes code for more than one transcript. Different ratios of transcripts of the same gene can be found in different cell types or states, indicating differential use of transcription start sites or differential splicing. Such differential transcript use (DTUs) events provide an additional layer of regulation and protein diversity. With the exceptions of PTPRC and CIITA, there are very few reported cases of DTU events in the immune system. To rigorously map DTUs between different human immune cell types, we leveraged four publicly available RNA sequencing datasets. We identified 282 DTU events between five human healthy immune cell types that appear in at least two datasets. The patterns of the DTU events were mostly cell-type-specific or lineage-specific, in the context of the five cell types tested. DTUs correlated with the expression pattern of potential regulators, namely, splicing factors and transcription factors. Of the several immune related conditions studied, only sepsis affected the splicing of more than a few genes and only in innate immune cells. Taken together, we map the DTUs landscape in human peripheral blood immune cell types, and present hundreds of genes whose transcript use changes between cell types or upon activation.

Original languageEnglish
Article number1116392
JournalFrontiers in Immunology
StatePublished - 30 Aug 2023


  • alternative promoters
  • alternative splicing
  • differential splicing
  • immune related diseases
  • immune system
  • sepsis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Mapping the splicing landscape of the human immune system'. Together they form a unique fingerprint.

Cite this