Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells

Oron Goldstein, Karin Meyer, Yariv Greenshpan, Nir Bujanover, Mili Feigin, Hadas Ner-Gaon, Tal Shay, Roi Gazit

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells.

Original languageEnglish
Pages (from-to)163-176
Number of pages14
JournalStem Cell Reports
Volume8
Issue number1
DOIs
StatePublished - 10 Jan 2017

Keywords

  • HSC
  • ImmGen
  • RNA-sequencing analysis
  • adult stem cell splicing
  • alternative splicing
  • hematopoietic stem cell
  • splicing-function
  • unannotated splicing
  • whole-transcriptome splicing

Fingerprint

Dive into the research topics of 'Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells'. Together they form a unique fingerprint.

Cite this