@article{ad856ee1f4a24c9287da811106df2971,
title = "Marker-free genetic manipulations in yeast using CRISPR/CAS9 system",
abstract = "The budding yeast is currently one of the major model organisms for the study of a wide variety of biological processes. Genetic manipulation of yeast involves the extensive usage of selectable markers that can lead to undesired effects. Thus, marker-free genetic manipulation in yeast is highly desirable for gene/promoter replacement and various other applications. Here we combine the power of selectable markers followed by CRISPR/CAS9 genome editing for common genetic manipulations in yeast in a marker-free manner. We demonstrate our approach for whole gene and promoter replacements and for high-efficiency operator array integration. Our approach allows the utilization of many thousands of existing strains including library strains for the generation of significant genetic changes in yeast in a marker-free and cloning-free fashion.",
keywords = "CRISPR/CAS9, Marker-free, S. cerevisiae, Yeast",
author = "Inga Soreanu and Adi Hendler and Danielle Dahan and Daniel Dovrat and Amir Aharoni",
note = "Funding Information: We thank all the members of the Aharoni?s lab for advices and support. This work was supported by the Israeli Science foundation (ISF) Grant numbers 2297/15 and 1340/17, Binational Science Foundation (BSF) Grant number 2013358 and the European research training network (ITN, Horizon 2020) ES-cat (722610). Funding Information: Acknowledgements We thank all the members of the Aharoni{\textquoteright}s lab for advices and support. This work was supported by the Israeli Science foundation (ISF) Grant numbers 2297/15 and 1340/17, Binational Science Foundation (BSF) Grant number 2013358 and the European research training network (ITN, Horizon 2020) ES-cat (722610). Publisher Copyright: {\textcopyright} 2018, Springer-Verlag GmbH Germany, part of Springer Nature.",
year = "2018",
month = oct,
day = "1",
doi = "10.1007/s00294-018-0831-y",
language = "English",
volume = "64",
pages = "1129--1139",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer Verlag",
number = "5",
}