Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells

Hila Naaman; Tatiana Rabinski; Avi Yizhak; Solly Mizrahi; Yonat Shemer Avni; Ran Taube; Bracha Rager; Yacov Weinstein; Glenn Rall; Jacob Gopas; Rivka Ofir

doi:10.1089/cell.2017.0034

Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells

Hila Naaman, Tatiana Rabinski, Avi Yizhak, Solly Mizrahi, Yonat Shemer Avni, Ran Taube, Bracha Rager, Yacov Weinstein, Glenn Rall, Jacob Gopas, Rivka Ofir

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.

Original language	English
Pages (from-to)	17-26
Number of pages	10
Journal	Cellular Reprogramming
Volume	20
Issue number	1
DOIs	https://doi.org/10.1089/cell.2017.0034
State	Published - 1 Feb 2018

Keywords

human-induced pluripotent stem cells
measles virus
persistent infection

ASJC Scopus subject areas

Biotechnology
Developmental Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/cell.2017.0034

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title = "Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells",

abstract = "In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.",

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author = "Hila Naaman and Tatiana Rabinski and Avi Yizhak and Solly Mizrahi and Avni, {Yonat Shemer} and Ran Taube and Bracha Rager and Yacov Weinstein and Glenn Rall and Jacob Gopas and Rivka Ofir",

note = "Funding Information: The authors acknowledge support from NIH R03 AI094164-01A1, the Richard H. Holzer Foundation, and the American Associates of the Ben-Gurion University (AABGU) the Philadelphia Chapter. Funding Information: The authors acknowledge support from NIH R03 AI094164-01A1, the Richard H. Holzer Foundation, and the American Associates of the Ben-Gurion University (AAB-GU) the Philadelphia Chapter. Publisher Copyright: {\textcopyright} Copyright 2018, Mary Ann Liebert, Inc. 2018.",

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AU - Naaman, Hila

AU - Rabinski, Tatiana

AU - Yizhak, Avi

AU - Mizrahi, Solly

AU - Avni, Yonat Shemer

AU - Taube, Ran

AU - Rager, Bracha

AU - Weinstein, Yacov

AU - Rall, Glenn

AU - Gopas, Jacob

AU - Ofir, Rivka

N1 - Funding Information: The authors acknowledge support from NIH R03 AI094164-01A1, the Richard H. Holzer Foundation, and the American Associates of the Ben-Gurion University (AABGU) the Philadelphia Chapter. Funding Information: The authors acknowledge support from NIH R03 AI094164-01A1, the Richard H. Holzer Foundation, and the American Associates of the Ben-Gurion University (AAB-GU) the Philadelphia Chapter. Publisher Copyright: © Copyright 2018, Mary Ann Liebert, Inc. 2018.

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N2 - In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.

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Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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