Measurement of circulating cell-free DNA levels by a new simple fluorescent test in patients with primary colorectal cancer

David Czeiger, Gad Shaked, Hadar Eini, Ilan Vered, Olga Belochitski, Avital Avriel, Samuel Ariad, Amos Douvdevani

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Elevated circulating cell-free DNA (CFD) levels were found in patients with cancer. The standard CFD assays are work-intensive and expensive. The aim was to evaluate in patients with cancer a new simple CFD assay. In mice inoculated with cancer cells, CFD levels correlated with tumor size. Compared with healthy subjects, 38 patients with colorectal cancer (CRC) had higher preoperative CFD levels (798 ± 409 vs 308 ± 256 ng/mL; P <.0001). Compared with patients free of disease at 1 year, CFD levels were elevated in patients who remained with disease or died (DD). CFD correlated with DD (P =.033), and a combined index of carcinoembryonic antigen × CFD exhibited a better correlation to DD than did pathologic staging (P =.0027 vs P =.0065). For patients with CRC, CFD levels were prognostic of death and disease. A large prospective study will need to be performed to truly evaluate the efficacy of this method for early detection, follow-up, and evaluation of patient response to treatment.

Original languageEnglish
Pages (from-to)264-270
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume135
Issue number2
DOIs
StatePublished - 1 Jan 2011
Externally publishedYes

Keywords

  • Biomarker
  • Carcinoembryonic antigen
  • Circulating cell-free DNA
  • Colorectal cancer
  • Pathologic grade

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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