Measurement of circulating cell-free DNA levels by a simple fluorescent test in patients with breast cancer

Ravit Agassi, David Czeiger, Gad Shaked, Avital Avriel, Jony Sheynin, Konstantin Lavrenkov, Samuel Ariad, Amos Douvdevani

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Objectives: To evaluate circulating cell-free DNA (CFD) measured by a simple fluorescent assay as a biomarker of breast cancer. Methods: We enrolled 38 patients with breast cancer before surgery, two patients with noncancerous breast lesions, nine patients after surgery, 16 healthy participants, and 29 control women admitted to the hospital emergency ward and released without hospitalization. CFD levels were measured by a direct fluorescence assay. Results: Presurgery patients with cancer had elevated CFD levels (1,010 ± 642 ng/mL), which were higher than those measured in the healthy control group (395 ± 248 ng/mL, P < .001), the noncancer breast lesion group (386 ± 40 ng/mL), the nonhospitalized control group (492 ± 193 ng/mL, P < .001), and the postsurgery cancer group (398 ± 162 ng/mL, P < .01). The area under the receiver operating characteristic curve of the presurgery vs healthy patient group was 0.83. CFD levels correlated with tumor size (P = .03, ρ = 0.36), nodal involvement (P = .0003, ρ = 0.56), and TNM stage (P = .0002, ρ = 0.56). All patients with axillary node involvement had a CFD value greater than 600 ng/mL. Conclusions: CFD measured using a simple fluorometric assay has shown good correlation to stage and enhanced sensitivity to locally advanced disease. A large prospective study is warranted to evaluate if inclusion of this method as a decisive marker before mammography is advantageous.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume143
Issue number1
DOIs
StatePublished - 1 Jan 2015
Externally publishedYes

Keywords

  • Biomarker
  • Breast cancer
  • CA 15-3
  • Circulating cell-free DNA (CFD)
  • Lymph node involvement

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