TY - JOUR
T1 - Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid
AU - Basavalingappa, Vasantha
AU - Bera, Santu
AU - Xue, Bin
AU - Azuri, Ido
AU - Tang, Yiming
AU - Tao, Kai
AU - Shimon, Linda J.W.
AU - Sawaya, Michael R.
AU - Kolusheva, Sofiya
AU - Eisenberg, David S.
AU - Kronik, Leeor
AU - Cao, Yi
AU - Wei, Guanghong
AU - Gazit, Ehud
N1 - Funding Information:
This work was supported in part by grants from the European Research Council under the European Union’s Horizon 2020 research and innovation program (BISON, Advanced ERC grant agreement ID:694426) (E.G.). V.B. gratefully acknowledges the Planning and Budget Committee, Israel, for financial support. Y.C. acknowledges the financial support from National Natural Science Foundation of China (Grant No. 11804148, 11804147). D.E. and M.S. acknowledge the Northeastern Collaborative Access Team beamline 24-ID-C, which is funded by the National Institute of General Medical Sciences from the National Institutes of Health (P30 GM124165). The Pilatus 6M detector on 24-ID-C beam line is funded by a NIH-ORIP HEI grant (S10 RR029205). This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. G.W. acknowledges the financial support from National Key Research and Development Program of China (2016YFA0501702) and the National Science Foundation of China (Grant No. 11674065). The authors thank Dr. Sigal Rencus-Lazar for language editing assistance, Dr. David Levy for PXRD and Dr. Alex Barbul for confocal microscopy. Dr. Roy Weinstain, School of Plant Sciences and Food Security, Tel Aviv University, for providing HPLC facility. Prof. Inna Slutsky and Dr. Samuel Frere, Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, for FLIM measurements.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamide-based molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m−1 and Young’s modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free “basket” formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications.
AB - The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamide-based molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m−1 and Young’s modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free “basket” formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications.
UR - http://www.scopus.com/inward/record.url?scp=85075435879&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-13250-x
DO - 10.1038/s41467-019-13250-x
M3 - Article
AN - SCOPUS:85075435879
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 5256
ER -