Mediated electrochemical detection of catechol by tyrosinase-based poly(dicarbazole) electrodes

Serge Cosnier, Sabine Szunerits, Robert S. Marks, Jean Paul Lellouche, Karine Perie

    Research output: Contribution to journalArticlepeer-review

    52 Scopus citations


    A new dicarbazole derivative functionalised by an N-hydroxysuccinimide group has been synthesised and electrochemically characterised. Upon oxidative electropolymerisation of this monomer in organic electrolytes, electroactive poly(dicarbazole) films were formed on platinum electrodes. The subsequent chemical grafting of tyrosinase on the poly(dicarbazole) film was easily performed by immersion in an enzymatic aqueous solution. The amperometric response of the resulting biosensors to catechol has been studied at -0.2 V vs. saturated calomel electrode (SCE). Since the reduction of quinone generates radicals which may induce electrode fouling, thionine, a phenothiazine dye, was covalently bound to the poly(dicarbazole) backbone as it mediates the reduction of quinoid products and therefore induces an enhancement of the performance of the tyrosinase-based biosensor.

    Original languageEnglish
    Pages (from-to)65-77
    Number of pages13
    JournalJournal of Biochemical and Biophysical Methods
    Issue number1
    StatePublished - 4 Dec 2001


    • Catechol
    • Dicarbazole
    • N-hydroxysuccinimide

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry


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