Membrane dynamic alterations associated with activation of human platelets by thrombin

I. Nathan; G. Fleisher; A. Dvilansky; A. Livne; A. H. Parola

doi:10.1016/0005-2736(80)90020-6

Membrane dynamic alterations associated with activation of human platelets by thrombin

I. Nathan, G. Fleisher, A. Dvilansky, A. Livne, A. H. Parola

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Two fluorescent probes, N-carboxymethylisatoic anhydride, which binds to membrane proteins, and 1,6-diphenyl-1,3,5-hexatriene, a lipophilic label, have been used to follow membrane microenvironmental changes. Activation of human platelets by thrombin resulted in a simultaneous increase in values of fluorescence polarization (P) of both probes during the stages of shape change and secretion, which further increased during platelet aggregation. The similar pattern of changes in P for both probes indicates the interdependence of lipids and proteins in the activated platelet membrane.

Original language	English
Pages (from-to)	417-421
Number of pages	5
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	598
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(80)90020-6
State	Published - 23 May 1980

Keywords

Aggregation
Fluorescence polarization
Membrane protein
Platelet
Secretion
Thrombin

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(80)90020-6

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title = "Membrane dynamic alterations associated with activation of human platelets by thrombin",

abstract = "Two fluorescent probes, N-carboxymethylisatoic anhydride, which binds to membrane proteins, and 1,6-diphenyl-1,3,5-hexatriene, a lipophilic label, have been used to follow membrane microenvironmental changes. Activation of human platelets by thrombin resulted in a simultaneous increase in values of fluorescence polarization (P) of both probes during the stages of shape change and secretion, which further increased during platelet aggregation. The similar pattern of changes in P for both probes indicates the interdependence of lipids and proteins in the activated platelet membrane.",

keywords = "Aggregation, Fluorescence polarization, Membrane protein, Platelet, Secretion, Thrombin",

author = "I. Nathan and G. Fleisher and A. Dvilansky and A. Livne and Parola, {A. H.}",

note = "Funding Information: This work was supported in part by a grant from the Head Scientist, Ministry of Health, Israel.",

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T1 - Membrane dynamic alterations associated with activation of human platelets by thrombin

AU - Nathan, I.

AU - Fleisher, G.

AU - Dvilansky, A.

AU - Livne, A.

AU - Parola, A. H.

N1 - Funding Information: This work was supported in part by a grant from the Head Scientist, Ministry of Health, Israel.

PY - 1980/5/23

Y1 - 1980/5/23

N2 - Two fluorescent probes, N-carboxymethylisatoic anhydride, which binds to membrane proteins, and 1,6-diphenyl-1,3,5-hexatriene, a lipophilic label, have been used to follow membrane microenvironmental changes. Activation of human platelets by thrombin resulted in a simultaneous increase in values of fluorescence polarization (P) of both probes during the stages of shape change and secretion, which further increased during platelet aggregation. The similar pattern of changes in P for both probes indicates the interdependence of lipids and proteins in the activated platelet membrane.

AB - Two fluorescent probes, N-carboxymethylisatoic anhydride, which binds to membrane proteins, and 1,6-diphenyl-1,3,5-hexatriene, a lipophilic label, have been used to follow membrane microenvironmental changes. Activation of human platelets by thrombin resulted in a simultaneous increase in values of fluorescence polarization (P) of both probes during the stages of shape change and secretion, which further increased during platelet aggregation. The similar pattern of changes in P for both probes indicates the interdependence of lipids and proteins in the activated platelet membrane.

KW - Aggregation

KW - Fluorescence polarization

KW - Membrane protein

KW - Platelet

KW - Secretion

KW - Thrombin

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JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

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ER -

Membrane dynamic alterations associated with activation of human platelets by thrombin

Abstract

Keywords

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