TY - JOUR
T1 - Membrane dynamic alterations associated with viral transformation and reversion. Decay of fluorescence emission and anisotropy studies of 3T3 cells
AU - Parola, Abraham H.
AU - Robbins, Phillips W.
AU - Blout, Elkan R.
N1 - Funding Information:
This work was supported,i n part, by NIH Grants AM07300 and 5-Rpl-CA14143-12a nd the NC1 Grants CA14142a nd CA14051. A. H. P. was a recipient of an NIH PostdoctoralF ellowship. A preliminaryr eport on this work was presenteda t the Meeting of the Israel Biochemical Society, April I, 1976B. eersheva,I srael.
PY - 1979/1/1
Y1 - 1979/1/1
N2 - Fluorescence lifetimes, anisotropies and rotational correlation time values of 1,6-diphenyl-1,3,5-hexatriene (DPH) in membranes of normal, transformed, and revertant 3T3 cells were determined by nanosecond (nsec), photon counting spectrofluorimetry. No change in lifetime values with transformation or reversion is observed. Fluorescence anisotropy decay curves show at least two components; an initial relatively fast decay and a non-zero "plateau" level component. The observed changes in the average anisotropy values, which qualitatively follow steady-state fluorescence polarization values, is due primarily to changes in the non-zero "plateau" level component. The anisotropy decay curves suggest that the rotational motion of the probe is restricted to a limited angular range. The present results are compared with model membrane systems.
AB - Fluorescence lifetimes, anisotropies and rotational correlation time values of 1,6-diphenyl-1,3,5-hexatriene (DPH) in membranes of normal, transformed, and revertant 3T3 cells were determined by nanosecond (nsec), photon counting spectrofluorimetry. No change in lifetime values with transformation or reversion is observed. Fluorescence anisotropy decay curves show at least two components; an initial relatively fast decay and a non-zero "plateau" level component. The observed changes in the average anisotropy values, which qualitatively follow steady-state fluorescence polarization values, is due primarily to changes in the non-zero "plateau" level component. The anisotropy decay curves suggest that the rotational motion of the probe is restricted to a limited angular range. The present results are compared with model membrane systems.
UR - http://www.scopus.com/inward/record.url?scp=0018409835&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(79)90598-6
DO - 10.1016/0014-4827(79)90598-6
M3 - Article
AN - SCOPUS:0018409835
SN - 0014-4827
VL - 118
SP - 205
EP - 214
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -