Abstract
Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to avoid its side effects on human and non-target organisms. In the present study, biologically active latex from Euphorbia heterophylla (Poinsettia) was utilized for synthesis of AgNPs. AgNPs was of spherical shape and narrow size range (20-50 nm). Occurrence of elemental silver and crystalline nature of AgNPs was analyzed. Role of latex metabolites in reduction and stabilization of AgNPs was analyzed by FT-IR, protein coagulation test and phytochemical analysis. Latex-synthesized AgNPs showed potential in selective and sensitive detection of toxic mercury ions (Hg2+) with limit of detection around 100 ppb. Addition of Hg2+ showed marked deviation in color and surface plasmon resonance spectra of AgNPs. Toxicity studies on aquatic non-target species Daphnia magna showed that latex-synthesized AgNPs (20.66 ± 1.52 % immobilization) were comparatively very less toxic than chemically synthesized AgNPs (51.66 ± 1.52 % immobilization). Similarly, comparative toxicity study on human red blood cells showed lower hemolysis (4.46 ± 0.01 %) by latex-synthesized AgNPs as compared to chemically synthesized AgNPs causing 6.14 ± 0.01 % hemolysis.
Original language | English |
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Pages (from-to) | 2223-2233 |
Number of pages | 11 |
Journal | Bioprocess and Biosystems Engineering |
Volume | 37 |
Issue number | 11 |
DOIs | |
State | Published - 10 Oct 2014 |
Externally published | Yes |
Keywords
- Daphnia magna
- Latex
- Mercury sensor
- Silver nanoparticles
- Toxicity
ASJC Scopus subject areas
- Biotechnology
- Bioengineering