Mercury sensing and toxicity studies of novel latex fabricated silver nanoparticles

Hemant P. Borase, Chandrashekhar D. Patil, Rahul B. Salunkhe, Rahul K. Suryawanshi, Bipinchandra K. Salunke, Satish V. Patil

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to avoid its side effects on human and non-target organisms. In the present study, biologically active latex from Euphorbia heterophylla (Poinsettia) was utilized for synthesis of AgNPs. AgNPs was of spherical shape and narrow size range (20-50 nm). Occurrence of elemental silver and crystalline nature of AgNPs was analyzed. Role of latex metabolites in reduction and stabilization of AgNPs was analyzed by FT-IR, protein coagulation test and phytochemical analysis. Latex-synthesized AgNPs showed potential in selective and sensitive detection of toxic mercury ions (Hg2+) with limit of detection around 100 ppb. Addition of Hg2+ showed marked deviation in color and surface plasmon resonance spectra of AgNPs. Toxicity studies on aquatic non-target species Daphnia magna showed that latex-synthesized AgNPs (20.66 ± 1.52 % immobilization) were comparatively very less toxic than chemically synthesized AgNPs (51.66 ± 1.52 % immobilization). Similarly, comparative toxicity study on human red blood cells showed lower hemolysis (4.46 ± 0.01 %) by latex-synthesized AgNPs as compared to chemically synthesized AgNPs causing 6.14 ± 0.01 % hemolysis.

Original languageEnglish
Pages (from-to)2223-2233
Number of pages11
JournalBioprocess and Biosystems Engineering
Volume37
Issue number11
DOIs
StatePublished - 10 Oct 2014
Externally publishedYes

Keywords

  • Daphnia magna
  • Latex
  • Mercury sensor
  • Silver nanoparticles
  • Toxicity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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