TY - CHAP
T1 - Metabolic Engineering and Synthetic Biology Approaches to Enhancing Production of Long-Chain Polyunsaturated Fatty Acids in Microalgae
AU - Khozin-Goldberg, Inna
AU - Sayanova, Olga
N1 - Funding Information:
I.K.G. acknowledges support by a grant from the Ministry of Science, Technology and Space, Israel. Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC), UK. This project was supported by BBSRC grant BB/L002957/1.
Publisher Copyright:
© Springer Nature Switzerland AG 2019.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Interest in microalgal biotechnology for the production of bioactive and nutritional ingredients has increased tremendously with the urgent need for developing renewable bioresources. Microalgae represent a huge and still insufficiently tapped resource of LC-PUFA for human nutrition and health-related applications. Photosynthetic omega-3 and omega-6 LC-PUFA-producing microalgae are potent organisms and synthetic biology chassis for the production of high-value constituents for both health and aquaculture sectors. This chapter covers the diversity of eukaryotic microalgae in relation to their LC-PUFA production and outlines the fundamental role of microalgae as primary producers of LC-PUFA. We provide mechanistic insights into the biosynthesis of LC-PUFA in photosynthetic microalgae and highlight their biotechnological applications. The ongoing research aims to understand the molecular mechanisms by which microalgae of different evolutionary groups synthesize, relocate and incorporate LC-PUFA into complex lipids in the context of their multifaceted cellular organization. This research is supported by the availability of genomic information and ‘omics’ studies in a growing number of species. Recent progress in microalgal genetic transformation, genome editing and metabolic engineering has enabled the manipulation of LC-PUFA biosynthesis. We discuss the prospects for LC-PUFA manufacturing by microalgal biotechnology as a renewable and sustainable alternative to the finite resources of LC-PUFA.
AB - Interest in microalgal biotechnology for the production of bioactive and nutritional ingredients has increased tremendously with the urgent need for developing renewable bioresources. Microalgae represent a huge and still insufficiently tapped resource of LC-PUFA for human nutrition and health-related applications. Photosynthetic omega-3 and omega-6 LC-PUFA-producing microalgae are potent organisms and synthetic biology chassis for the production of high-value constituents for both health and aquaculture sectors. This chapter covers the diversity of eukaryotic microalgae in relation to their LC-PUFA production and outlines the fundamental role of microalgae as primary producers of LC-PUFA. We provide mechanistic insights into the biosynthesis of LC-PUFA in photosynthetic microalgae and highlight their biotechnological applications. The ongoing research aims to understand the molecular mechanisms by which microalgae of different evolutionary groups synthesize, relocate and incorporate LC-PUFA into complex lipids in the context of their multifaceted cellular organization. This research is supported by the availability of genomic information and ‘omics’ studies in a growing number of species. Recent progress in microalgal genetic transformation, genome editing and metabolic engineering has enabled the manipulation of LC-PUFA biosynthesis. We discuss the prospects for LC-PUFA manufacturing by microalgal biotechnology as a renewable and sustainable alternative to the finite resources of LC-PUFA.
UR - http://www.scopus.com/inward/record.url?scp=85101556544&partnerID=8YFLogxK
U2 - 10.1007/978-3-030-25233-5_7
DO - 10.1007/978-3-030-25233-5_7
M3 - Chapter
AN - SCOPUS:85101556544
T3 - Grand Challenges in Biology and Biotechnology
SP - 249
EP - 289
BT - Grand Challenges in Biology and Biotechnology
PB - Springer Science and Business Media B.V.
ER -
