TY - JOUR
T1 - Metabolic response of Botryococcus braunii to high bicarbonate dosages and other conditions
T2 - analysis of photosynthetic performance, productivity, and lipidomic profile
AU - Giraldo, Néstor David
AU - Correa, Sandra Marcela
AU - Arbeláez, Andrés
AU - Figueroa, Felix L.
AU - Ríos-Estepa, Rigoberto
AU - Atehortúa, Lucía
N1 - Funding Information:
This work was supported by the Agencia Nacional de Hidrocarburos (ANH), Ministerio de Ciencia, Tecnología e Innovación Colciencias and Universidad de Antioquia under the grant 721–2015 “Convocatoria para la formación de recurso humano en Colombia en el área de hidrocarburos, a través de proyectos de investigación año 2015”.
Funding Information:
The authors thank ?nne Michaelis, Aleksandra Skirycz, and Yariv Brotman (Max Planck Institute of Molecular Plant Physiology?Potsdam, Germany) for their support with the lipid analysis.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/10/1
Y1 - 2021/10/1
N2 - In this study the metabolic responses of Botryococcus braunii were analyzed upon different inorganic carbon dosages and nutrient limitation conditions in terms of lipid and biomass productivity, as well as photosynthetic performance. The nutritional schemes evaluated included different levels of sodium bicarbonate and nitrogen and phosphorus starvation, which were contrasted against standard cultures fed with CO2. Bicarbonate was found to be an advantageous carbon source since high dosages caused a significant increase in biomass and lipid productivity, in addition to an enhanced photosynthetic quantum yield and neutral lipids abundance. This contrasts to the commonly used approach of microalgae nutrient limitation, which leads to high lipid accumulation at the expense of impaired cellular growth, causing a decline in overall lipid productivity. The lipidome analysis served to hypothesize about the influence of the nutritional context on B. braunii structural and storage lipid metabolism, besides the adaptive responses exhibited by cells that underwent nutrient stress.
AB - In this study the metabolic responses of Botryococcus braunii were analyzed upon different inorganic carbon dosages and nutrient limitation conditions in terms of lipid and biomass productivity, as well as photosynthetic performance. The nutritional schemes evaluated included different levels of sodium bicarbonate and nitrogen and phosphorus starvation, which were contrasted against standard cultures fed with CO2. Bicarbonate was found to be an advantageous carbon source since high dosages caused a significant increase in biomass and lipid productivity, in addition to an enhanced photosynthetic quantum yield and neutral lipids abundance. This contrasts to the commonly used approach of microalgae nutrient limitation, which leads to high lipid accumulation at the expense of impaired cellular growth, causing a decline in overall lipid productivity. The lipidome analysis served to hypothesize about the influence of the nutritional context on B. braunii structural and storage lipid metabolism, besides the adaptive responses exhibited by cells that underwent nutrient stress.
KW - Biofuels
KW - Biorefinery
KW - Botryococcus braunii
KW - Chlorophyceae
KW - Lipidomics
KW - Photosynthesis
UR - http://www.scopus.com/inward/record.url?scp=85112572488&partnerID=8YFLogxK
U2 - 10.1007/s10811-021-02544-7
DO - 10.1007/s10811-021-02544-7
M3 - Article
AN - SCOPUS:85112572488
SN - 0921-8971
VL - 33
SP - 2875
EP - 2896
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
IS - 5
ER -