Metabolomic Analysis of Natural Variation in Arabidopsis

Si Wu, Saleh Alseekh, Yariv Brotman, Alisdair R. Fernie

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Methodological advances in coupled-mass spectrometry (gas chromatography and liquid chromatography; GC-MS and LC-MS) have rendered the profiling of highly complex plant extracts relatively facile and allowed that their high-throughput use aids the investigation of a range of biological questions. Among these is the elucidation of the genetic factors underlying metabolite abundance. For this purpose genome-wide association studies (GWAS) are being widely adopted in Arabidopsis with the resultant quantitative trait loci being subjected to cross-validation by the use of recombinant inbred lines, introgression lines, and T-DNA insertional knockout lines.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages19
StatePublished - 1 Jan 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Gas-chromatography mass-spectrometry
  • Genome-wide association mapping
  • Liquid-chromatography mass-spectrometry
  • Metabolomics
  • QTL analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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