Metagenomic analysis for profiling of microbial communities and tolerance in metal-polluted pulp and paper industry wastewater

Pooja Sharma, Sonam Tripathi, Ram Chandra

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

This work aimed to study the profiling and efficiency of microbial communities and their abundance in the pulp and paper industry wastewater, which contained toxic metals, high biological oxygen demands, chemical oxygen demand, and ions contents. Sequence alignment of the 16S rRNA V3-V4 variable region zone with the Illumina MiSeq framework revealed 25356 operating taxonomical units (OTUs) derived from the wastewater sample. The major phyla identified in wastewater were Proteobacteria, Bacteroidetes, Firmicutes, Chloroflexi, Actinobacteria, Spirochetes, Patesibacteria, Acidobacteria, and others including unknown microbes. The study showed the function of microbial communities essential for the oxidation and detoxifying of complex contaminants and design of effective remediation techniques for the re-use of polluted wastewater. Findings demonstrated that the ability of different classes of microbes to adapt and survive in metal-polluted wastewater irrespective of their relative distribution, as well as further attention can be provided to its use in the bioremediation process.

Original languageEnglish
Article number124681
JournalBioresource Technology
Volume324
DOIs
StatePublished - 1 Mar 2021
Externally publishedYes

Keywords

  • Biological wastewater treatment
  • Illumina MiSeq sequencing
  • Microbial community
  • Operational taxonomic unit
  • Wastewater treatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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