Method for the generation of induced hematopoietic stem cells

Leonid Olender, Klil Levy, Roi Gazit

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Reprogramming proved the possibility to change cell identity by transient overexpression of defined transcription factors. However, the efficiencies of pioneer protocols are extremely low, and mechanistic understanding is still under intensive research. Hematopoietic stem cells (HSCs) are prototypic adult stem cells, leading scientific research and clinical applications. We had reported the possibility of direct reprogramming of blood cells into induced-HSCs. In this chapter, we detail the protocol and elaborate information on critical steps. From the identification of candidate factors, through cloning and lentiviral production, this protocol can help anyone interested in reprogramming toward the adult stem cell state. A detailed protocol should enable new ideas to realize and further open new frontiers for adult stem cell research.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 1 Jan 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Induced hematopoietic stem cells
  • Induced pluripotent stem cells
  • Lentiviral vectors
  • Reprogramming

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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