Methods to study the role of progranulin in the tumor microenvironment

Moshe Elkabets, Samuel Brook

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Accurate measurement of progranulin (PGRN) in the circulation and in the tumor microenvironment is essential for understanding its role in cancer progression and metastasis. This chapter describes a number of approaches to measure the transcription level of the GRN gene and to detect and analyze PGRN expression in cancer cells and in the local environment of the tumor, in mouse and human samples. These validated protocols are utilized to investigate the functional role of PGRN in cancer. Finally, we discuss strategies to investigate the functions of PGRN in tumors using genetically modified mouse models and gene silencing techniques.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-176
Number of pages22
DOIs
StatePublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1806
ISSN (Print)1064-3745

Keywords

  • ELISA
  • Flow cytometry
  • GRN
  • Immunofluorescence
  • Immunohistochemistry
  • Progranulin
  • Tumor microenvironment
  • qPCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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