TY - JOUR
T1 - Methyl farnesoate in the prawn Macrobrachium rosenbergii
T2 - Synthesis by the mandibular organ in vitro, and titers in the hemolymph
AU - Sagi, Amir
AU - Homola, Ellen
AU - Laufer, Hans
N1 - Funding Information:
Acknowledgements--We thank Dr John Glude and Mr Dennis Delong of Aquaculture Enterprises, Inc. Sabana Grande, Puerto Rico, for selecting and providing the prawns used during this study. We thank P.D.M. and Associates, Norwich, CT for providing pelleted prawn food. We thank Dr Frank Mauri from the Biotechnology Center of the University of Connecticut for his technical assistance and advice. We thank Mary Jane Spring for the artistic rendition of the mandibular organ. This study was supported by a U.S. Israel Binational Agricultural Research and Development fellowship and by a Fulbright fellowship (to A.S.) and the Sea Grant College program (NOAA), the Lady Davis Trust and a National Research Service Award from the NIH (to H.L.)
PY - 1991/1/1
Y1 - 1991/1/1
N2 - 1. 1. The mandibular organ of Macrobrachium rosenbergii was identified and cultured in the presence of [methyl-3H]-methionine. 2. 2. Radiolabelled methyl farnesoate was extracted and quantitated from cultured glands and culture media of both males and females: variation in synthesis rates was observed (1558-52,652 DPM/hr per gland) between individual prawns. 3. 3. No significant incorporation of isotope into hexane-extractable material was observed in any other tissue cultured. 4. 4. The concentration of methyl farnesoate in the hemolymph of M. rosenbergii was determined using normal phase HPLC with cis-trans (non-biological isomer) as an internal standard. 5. 5. Hemolymph from males contained 17.3 ± 9.5 ng/ml methyl farnesoate. 6. 6. Female hemolymph contained 24 ± 13.1 ng/ml methyl farnesoate.
AB - 1. 1. The mandibular organ of Macrobrachium rosenbergii was identified and cultured in the presence of [methyl-3H]-methionine. 2. 2. Radiolabelled methyl farnesoate was extracted and quantitated from cultured glands and culture media of both males and females: variation in synthesis rates was observed (1558-52,652 DPM/hr per gland) between individual prawns. 3. 3. No significant incorporation of isotope into hexane-extractable material was observed in any other tissue cultured. 4. 4. The concentration of methyl farnesoate in the hemolymph of M. rosenbergii was determined using normal phase HPLC with cis-trans (non-biological isomer) as an internal standard. 5. 5. Hemolymph from males contained 17.3 ± 9.5 ng/ml methyl farnesoate. 6. 6. Female hemolymph contained 24 ± 13.1 ng/ml methyl farnesoate.
UR - http://www.scopus.com/inward/record.url?scp=0025871371&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(91)90157-9
DO - 10.1016/0305-0491(91)90157-9
M3 - Article
AN - SCOPUS:0025871371
SN - 0305-0491
VL - 99
SP - 879
EP - 882
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
IS - 4
ER -
