Methyl farnesoate in the prawn Macrobrachium rosenbergii: Synthesis by the mandibular organ in vitro, and titers in the hemolymph

Amir Sagi, Ellen Homola, Hans Laufer

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

1. 1. The mandibular organ of Macrobrachium rosenbergii was identified and cultured in the presence of [methyl-3H]-methionine. 2. 2. Radiolabelled methyl farnesoate was extracted and quantitated from cultured glands and culture media of both males and females: variation in synthesis rates was observed (1558-52,652 DPM/hr per gland) between individual prawns. 3. 3. No significant incorporation of isotope into hexane-extractable material was observed in any other tissue cultured. 4. 4. The concentration of methyl farnesoate in the hemolymph of M. rosenbergii was determined using normal phase HPLC with cis-trans (non-biological isomer) as an internal standard. 5. 5. Hemolymph from males contained 17.3 ± 9.5 ng/ml methyl farnesoate. 6. 6. Female hemolymph contained 24 ± 13.1 ng/ml methyl farnesoate.

Original languageEnglish
Pages (from-to)879-882
Number of pages4
JournalComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume99
Issue number4
DOIs
StatePublished - 1 Jan 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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